Anti-EGFR Antibody (5V479) is a Mouse antibody targeting EGFR. Anti-EGFR Antibody (5V479) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.

IgG

5V479

Human

1. Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (EGFR) Monoclonal Antibody, Unconjugated (TMAB-05468) at 1:400 overnight at 4°C, followed by a conjugated Goat Anti-Mouse IgG antibody for 90 minutes, and DAPI for nuclei staining.
2. Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (EGFR) Monoclonal Antibody, Unconjugated (TMAB-05468) at 1:400 overnight at 4°C, followed by a conjugated Goat Anti-Mouse IgG antibody for 90 minutes, and DAPI for nuclei staining.
3. Tissue/cell: Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (EGFR) monoclonal Antibody, Unconjugated (TMAB-05468) 1:100, 90 minutes at 37°C; followed by a CY3 conjugated Goat Anti-Mouse IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.
4. 25 μg total protein per lane of various lysates (see on figure) probed with EGFR monoclonal antibody, unconjugated (TMAB-05468) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
5. 25 μg total protein per lane of various lysates (see on figure) probed with EGFR monoclonal antibody, unconjugated (TMAB-05468) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.

WB: 1:1000-5000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; ICC/IF: 1:100-500; IF: 1:100-500

Monoclonal

KLH conjugated synthetic peptide: human EGFR

Human

EGFR

Epidermal growth factor receptor

Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling. Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin.
Isoform 2 may act as an antagonist of EGF action.