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Anti-EGFR Antibody (5D853)
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Catalog No. TMAY-00022
Alias PIG61, NISBD2, mENA, HER1, ERBB1, ERBB, epidermal growth factor receptor
Anti-EGFR Antibody (5D853) is a Mouse antibody targeting EGFR. Anti-EGFR Antibody (5D853) can be used in FCM,ICC/IF.
Anti-EGFR Antibody (5D853)
Anti-EGFR Antibody (5D853)
😃Good
Catalog No. TMAY-00022Alias PIG61, NISBD2, mENA, HER1, ERBB1, ERBB, epidermal growth factor receptor
Anti-EGFR Antibody (5D853) is a Mouse antibody targeting EGFR. Anti-EGFR Antibody (5D853) can be used in FCM,ICC/IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 20 μL | $69 | 7-10 days | |
| 100 μL | $198 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Anti-EGFR Antibody (5D853) is a Mouse antibody targeting EGFR. Anti-EGFR Antibody (5D853) can be used in FCM,ICC/IF. |
| Alias | PIG61, NISBD2, mENA, HER1, ERBB1, ERBB, epidermal growth factor receptor |
| Ig Type | Monoclonal Mouse IgG1 |
| Clone | 5D853 |
| Reactivity | Human |
| Verified Activity | 1. Immunofluorescence staining of EGFR in A431 cells. Cells were fixed with 4% PFA,blocked with 10% serum, and incubated with mouse anti-Human EGFR monoclonal antibody (dilution ratio 1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cell membrane. 2. Flow cytometric analysis of Human EGFR expression on A431 cells. Cells were stained with purified anti-Human EGFR, then a FITC-conjugated second step antibody. The histogram were derived from gated events with the forward and side light-scatter characteristics of intact cells. |
| Application | FCM,ICC/IF |
| Recommended Dose | ICC-IF: 1:20-1:100; FCM: 1:25-1:100 |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Construction | This product is a recombinant monoclonal antibody expressed from HEK293 cells. |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | As a member of the epidermal growth factor receptor (EGFR) family, EGFR protein is type I transmembrane glycoprotein that binds a subset of EGF family ligands including EGF, amphiregulin, TGF-α, betacellulin, etc. EGFR protein plays a crucial role in signaling pathway in the regulation of cell proliferation, survival and differentiation. Binding of a ligand induces EGFR protein homo- or heterodimerization, the subsequent tyrosine autophosphorylation and initiates various down stream pathways (MAPK, PI3K/PKB and STAT). In addition, EGFR signaling also has been shown to exert action on carcinogenesis and disease progression, and thus EGFR protein is proposed as a target for cancer therapy currently.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy |
| Conjucates | Unconjugated |
| Others Formats | APC/FITC/Biotin/PE |
| Antibody Types Available | 5 |
| Immunogen | Recombinant Human EGFR / HER1 / ErbB1 protein (TMPY-00742) |
| Antigen Species | Human |
| Biology Area | Cancer Drug Targets, Receptor Tyrosine Kinases (RTKs) |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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