Anti-EGF Polyclonal Antibody is a Rabbit antibody targeting EGF. Anti-EGF Polyclonal Antibody can be used in ICC/IF,FCM.

IgG

Human

1. Blank control: K562. Primary Antibody (green line): Rabbit Anti-EGF antibody (TMAB-00591)
Dilution: 1 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-AF488
Dilution: 1 μg/test.
Protocol
The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.
2. U87MG cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (EGF) polyclonal Antibody, Unconjugated (TMAB-00591) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nucleus.

ICC/IF,FCM

ICC/IF: 1:100; FCM: 1ug/Test

Polyclonal

KLH conjugated synthetic peptide: human EGF

Human

EGF,Cell differentiation,Growth factors and hormones ELISA kits,EGF