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Anti-EEF1G Polyclonal Antibody

Catalog No. TMAB-05451 Copy Product Info
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Anti-EEF1G Polyclonal Antibody is a Rabbit antibody targeting EEF1G. Anti-EEF1G Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

Anti-EEF1G Polyclonal Antibody

Copy Product Info
🥰Excellent
Catalog No. TMAB-05451

Anti-EEF1G Polyclonal Antibody is a Rabbit antibody targeting EEF1G. Anti-EEF1G Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

Anti-EEF1G Polyclonal Antibody
Pack SizePriceUSA StockGlobal StockQuantity
50 μL$2217-10 days7-10 days
100 μL$3747-10 days7-10 days
200 μL$5287-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Description
Anti-EEF1G Polyclonal Antibody is a Rabbit antibody targeting EEF1G. Anti-EEF1G Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Ig Type
IgG
Reactivity
Human,Mouse,Rat (predicted:Dog,Pig,Cow,Horse,Rabbit)
Verified Activity
1. Sample: Cerebrum (Mouse) Lysate at 40 μg
Primary: Anti-EEF1G (TMAB-05451) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 50 kD
Observed band size: 50 kD
2. Sample: Hela Cell (Human) Lysate at 40 μg
Primary: Anti-EEF1G (TMAB-05451) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 50 kD
Observed band size: 50 kD
3. Paraformaldehyde-fixed, paraffin embedded (human pancreatic cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (EEF1G) Polyclonal Antibody, Unconjugated (TMAB-05451) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (EEF1G) Polyclonal Antibody, Unconjugated (TMAB-05451) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
Application
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Tissue SpecificityHighly expressed in pancreatic tumor tissue and to a lesser extent in normal kidney, intestine, pancreas, stomach, lung, brain, spleen and liver.
ConstructionPolyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Research BackgroundEF-1 (elongation factor-1) is a multi-protein complex that is responsible for the delivery of aminoacyl-tRNAs to the ribosome. EF-1 gamma (elongation factor 1-gamma), also known as EEF1G or GIG35, is a 437 amino acid subunit of the EF-1 complex. Expressed in stomach, pancreas, brain, lung, kidney, intestine, liver and spleen, EF-1 gamma contains an N-terminal glutathione transferase domain which is thought to be involved in anchoring the complex to various cellular components. Additionally, EF-1 gamma may play a key role in the assembly of multiprotein complexes containing aminoacyl-tRNA synthetases. Increased expression of EF-1 gamma is associated with pancreatic cancer, suggesting a possible role for EF-1 gamma in the oncogenic transformation process.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human EEF1G
Antigen Species
Human
Gene Name
EEF1G
Gene ID
Protein Name
Elongation factor 1-gamma
Uniprot ID
Function
Probably plays a role in anchoring the complex to other cellular components.
Chemical Properties
Molecular WeightTheoretical: 50 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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