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Anti-eEF1A2 Polyclonal Antibody
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Catalog No. TMAB-05449
Anti-eEF1A2 Polyclonal Antibody is a Rabbit antibody targeting eEF1A2. Anti-eEF1A2 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-eEF1A2 Polyclonal Antibody
Anti-eEF1A2 Polyclonal Antibody
Copy Product Info😃Good
Catalog No. TMAB-05449
Anti-eEF1A2 Polyclonal Antibody is a Rabbit antibody targeting eEF1A2. Anti-eEF1A2 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $221 | 7-10 days | 7-10 days | |
| 100 μL | $371 | 7-10 days | 7-10 days | |
| 200 μL | $529 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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| Description | Anti-eEF1A2 Polyclonal Antibody is a Rabbit antibody targeting eEF1A2. Anti-eEF1A2 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| Reactivity | Mouse,Rat (predicted:Human,Zebrafish) |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (eEF1A2) Polyclonal Antibody, Unconjugated (TMAB-05449) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining. 2. 25 μg total protein per lane of various lysates (see on figure) probed with eEF1A2 polyclonal antibody, unconjugated (TMAB-05449) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min. |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Nucleus. |
| Tissue Specificity | Brain, heart, and skeletal muscle. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | Elongation factor 1 alpha 2 (EF-1 alpha2) is a eukaryotic protein translation factor that is expressed in terminally differentiated cells of skeletal muscle, heart, and certain areas of the brain. Along with protein synthesis, EF-1 alpha2 also plays a role in cytoskeletal remodelling and apoptosis. The gene encoding for EF-1 alpha2 maps to chromosome 20q13. Wasted (wst) refers to a spontaneous autosomal recessive mutation in which this gene is deleted, and it leads to tremors and disturbances of gait shortly after weaning, followed by motor neuron degeneration, paralysis, and death by about 28 days in mice. EF-1 alpha2 has the ability to transform mammalian cells and is highly expressed in tumors of the ovary, breast, and lung, thereby proving a good candidate for an oncogene. In addition to this, EF-1 alpha2 enhances focus formation, allows anchorage-independent growth, and decreases the doubling time of rodent fibroblasts. |
| Immunogen | KLH conjugated synthetic peptide: human eEF1A2 |
| Antigen Species | Human |
| Gene Name | EEF1A2 |
| Gene ID | |
| Protein Name | Elongation factor 1-alpha 2 |
| Uniprot ID | |
| Function | This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. |
| Molecular Weight | Theoretical: 50 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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