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Anti-EEF1A1 Polyclonal Antibody

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Catalog No. TMAB-05446

Anti-EEF1A1 Polyclonal Antibody is a Rabbit antibody targeting EEF1A1. Anti-EEF1A1 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.

Anti-EEF1A1 Polyclonal Antibody

Anti-EEF1A1 Polyclonal Antibody

Copy Product Info
😃Good
Catalog No. TMAB-05446
Anti-EEF1A1 Polyclonal Antibody is a Rabbit antibody targeting EEF1A1. Anti-EEF1A1 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.
Pack SizePriceUSA WarehouseGlobal WarehouseQuantity
50 μL$2227-10 days7-10 days
100 μL$3727-10 days7-10 days
200 μL$5277-10 days7-10 days
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction

Bioactivity
Description
Anti-EEF1A1 Polyclonal Antibody is a Rabbit antibody targeting EEF1A1. Anti-EEF1A1 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.
Ig Type
IgG
Reactivity
Mouse,Rat (predicted:Human,Chicken,Dog,Pig,Cow,Horse,Rabbit)
Verified Activity
1. Tissue/cell: Mouse embryos; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-EF1a/eEF1A1 Polyclonal Antibody, Unconjugated (TMAB-05446) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
2. Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-eEF1A1 Polyclonal Antibody, Unconjugated (TMAB-05446) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
Application
Recommended Dose
IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationCytoplasm.
Tissue SpecificityBrain, placenta, lung, liver, kidney, pancreas but barely detectable in heart and skeletal muscle
ConstructionPolyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Research BackgroundThis gene encodes an isoform of the alpha subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This isoform (alpha 1) is expressed in brain, placenta, lung, liver, kidney, and pancreas, and the other isoform (alpha 2) is expressed in brain, heart and skeletal muscle. This isoform is identified as an autoantigen in 66% of patients with Felty syndrome. This gene has been found to have multiple copies on many chromosomes, some of which, if not all, represent different pseudogenes. [provided by RefSeq, Jul 2008]
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human EEF1A1
Antigen Species
Human
Gene Name
EEF1A1
Gene ID
Protein Name
Elongation factor 1-alpha 1
Uniprot ID
Function
This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.
Chemical Properties
Molecular WeightTheoretical: 50 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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