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Anti-EEF1A1 Antibody (9P202)
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Catalog No. TMAB-05447
Anti-EEF1A1 Antibody (9P202) is a Mouse antibody targeting EEF1A1. Anti-EEF1A1 Antibody (9P202) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.
Anti-EEF1A1 Antibody (9P202)
Anti-EEF1A1 Antibody (9P202)
Copy Product Info😃Good
Catalog No. TMAB-05447
Anti-EEF1A1 Antibody (9P202) is a Mouse antibody targeting EEF1A1. Anti-EEF1A1 Antibody (9P202) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 25 μL | $148 | 7-10 days | 7-10 days | |
| 50 μL | $262 | 7-10 days | 7-10 days | |
| 100 μL | $472 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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| Description | Anti-EEF1A1 Antibody (9P202) is a Mouse antibody targeting EEF1A1. Anti-EEF1A1 Antibody (9P202) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF. |
| Ig Type | IgG1, Kappa |
| Clone | 9P202 |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Cell line: MCF7 Fixative: 100% Ice-cold methanol Permeabilization: 0.1% TritonX-100 Primary ab dilution: 1:50 Primary incubation condition: 4°C overnight Secondary ab: Goat Anti-Mouse IgG Nuclear counter stain: DAPI (Blue) Comment: Color green is the positive signal for TMAB-05447 2. Blocking buffer: 5% NFDM/TBST Primary ab dilution: 1:1000 Primary ab incubation condition: 2 hours at room temperature Secondary ab: Goat Anti-Mouse IgG H&L (HRP) Lysate: MCF-7, Daudi, 4T1, BRL Protein loading quantity: 20 μg Exposure time: 1 s Predicted MW: 50 kDa Observed MW: 50 kDa 3. Blocking buffer: 5% NFDM/TBST Primary ab dilution: 1:1000 Primary ab incubation condition: 2 hours at room temperature Secondary ab: Goat Anti-Mouse IgG H&L (HRP) Lysate: MCF-7, Daudi, 4T1, BRL Protein loading quantity: 20 μg Exposure time: 1 s Predicted MW: 50 kDa Observed MW: 50 kDa 4. Tissue: Rat liver Section type: Formalin fixed & Paraffin-embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:100 Primary ab incubation condition: 1 hour at room temperature Secondary ab: SP Kit (Mouse) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for TMAB-05447 5. Tissue: Human breast cancer Section type: Formalin fixed & Paraffin-embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:100 Primary ab incubation condition: 1 hour at room temperature Secondary ab: SP Kit (Mouse) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for TMAB-05447 6. Tissue: Mouse brain Section type: Formalin fixed & Paraffin-embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:100 Primary ab incubation condition: 1 hour at room temperature Secondary ab: SP Kit (Mouse) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for TMAB-05447 |
| Application | |
| Recommended Dose | WB: 1:500-1000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; ICC/IF: 1:100-500; IF: 1:100-500 |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Subcellular Localization | Cytoplasm. |
| Tissue Specificity | Brain, placenta, lung, liver, kidney, pancreas but barely detectable in heart and skeletal muscle |
| Construction | Recombinant Antibody |
| Purification | Protein G purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | This gene encodes an isoform of the alpha subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This isoform (alpha 1) is expressed in brain, placenta, lung, liver, kidney, and pancreas, and the other isoform (alpha 2) is expressed in brain, heart and skeletal muscle. This isoform is identified as an autoantigen in 66% of patients with Felty syndrome. This gene has been found to have multiple copies on many chromosomes, some of which, if not all, represent different pseudogenes. [provided by RefSeq, Jul 2008] |
| Gene Name | EEF1A1 |
| Gene ID | |
| Protein Name | Elongation factor 1-alpha 1 |
| Function | This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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