Anti-EEF1A1 Antibody (5C318) is a Rabbit antibody targeting EEF1A1. Anti-EEF1A1 Antibody (5C318) can be used in ICC/IF, IF, IHC-Fr, IHC-P, WB.

IgG

5C318

Human,Mouse,Rat

1. Paraformaldehyde-fixed, paraffin embedded Mouse Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with EEF1A1 Monoclonal Antibody, Unconjugated (TMAB-05448) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with EEF1A1 Monoclonal Antibody, Unconjugated (TMAB-05448) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with EEF1A1 Monoclonal Antibody, Unconjugated (TMAB-05448) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with EEF1A1 Monoclonal Antibody, Unconjugated (TMAB-05448) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with EEF1A1 Monoclonal Antibody, Unconjugated (TMAB-05448) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
6. Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with EEF1A1 Monoclonal Antibody, Unconjugated (TMAB-05448) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
7. Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with EEF1A1 Monoclonal Antibody, Unconjugated (TMAB-05448) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
8. Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with EEF1A1 Monoclonal Antibody, Unconjugated (TMAB-05448) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
9. Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with EEF1A1 Monoclonal Antibody, Unconjugated (TMAB-05448) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
10. 4% Paraformaldehyde-fixed PANC-1 (H) cell; Triton X-100 at r. T. for 20 min; Antibody incubation with (EEF1A1) monoclonal Antibody, unconjugated (TMAB-05448) 1: 100, 90 min at 37°C; followed by BF488 conjugated Goat Anti-Rabbit IgG antibody (green, BF488) at 37°C for 90 min, DAPI (blue) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.

ICC/IF=1:50-200; IF=1:50-200; IHC-Fr=1:50-200; IHC-P=1:50-200; WB=1:500-2000

Monoclonal

KLH conjugated synthetic peptide: human eEF1a1

Human

EEF1A1

Elongation factor 1-alpha 1

This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.