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Anti-EEA1 Antibody (2X782)
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Catalog No. TMAC-01228
Alias Zinc finger FYVE domain-containing protein 2, ZFYVE2, MSTP105, MST105, Endosome-associated protein p162, EEA1, EEA 1, Early endosome associated protein, Early endosome antigen 1, 162kD, Early endosome antigen 1
Anti-EEA1 Antibody (2X782) is a Mouse antibody targeting EEA1. Anti-EEA1 Antibody (2X782) can be used in WB,ICC,IHC.
Anti-EEA1 Antibody (2X782)
Anti-EEA1 Antibody (2X782)
😃Good
Catalog No. TMAC-01228Alias Zinc finger FYVE domain-containing protein 2, ZFYVE2, MSTP105, MST105, Endosome-associated protein p162, EEA1, EEA 1, Early endosome associated protein, Early endosome antigen 1, 162kD, Early endosome antigen 1
Anti-EEA1 Antibody (2X782) is a Mouse antibody targeting EEA1. Anti-EEA1 Antibody (2X782) can be used in WB,ICC,IHC.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $297 | 7-10 days | |
| 100 μL | $427 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Anti-EEA1 Antibody (2X782) is a Mouse antibody targeting EEA1. Anti-EEA1 Antibody (2X782) can be used in WB,ICC,IHC. |
| Synonyms | Zinc finger FYVE domain-containing protein 2, ZFYVE2, MSTP105, MST105, Endosome-associated protein p162, EEA1, EEA 1, Early endosome associated protein, Early endosome antigen 1, 162kD, Early endosome antigen 1 |
| Clone | 2X782 |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Western blot analysis on 293 cell lysates using anti-EEA1 mouse mAb. 2. ICC staining EEA1 in Hela cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 3. ICC staining EEA1 in A431 cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
| Application | |
| Recommended Dose | WB: 1:2000-5000; ICC: 1:100 |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Construction | Hybridoma Monoclonal Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | EEA1 localizes exclusively to early endosomes?and has an important role in endosomal trafficking. EEA1 binds directly to the phospholipid phosphatidylinositol 3-phosphate?through its C-terminalFYVE domain?and forms a homodimer?through a?coiled coil. EEA1 acts as a tethering molecule that couples vesicle docking with SNAREs such as?N-ethylmaleimide sensitive fusion protein, bringing the endosomes physically closer and ultimately resulting in the fusion and delivery of endosomal cargo. Due to the proteins importance in vesicular trafficking, a number of intracellular bacteria prevent EEA1 recruitment to the vacuole.Mycobacterium tuberculosis?is known to inhibit the recruitment of EEA1 to the phagosomal membrane through CamKII. Legionella pneumophila?also prevents EEA1 recruitment through a currently unknown mechanism.[5] Interestingly, the related pathogen Legionella longbeachae?recruits EEA1 and appears to replicate within a modified early endosome. |
| Conjucates | Unconjugated |
| Immunogen | Peptide |
| Uniprot ID |
| Molecular Weight | Theoretical: 162 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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