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Anti-EDG3 Polyclonal Antibody
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Catalog No. TMAB-00589
Alias Sphingosine 1 phosphate receptor Edg3, Sphingosine 1 phosphate receptor Edg 3, Sphingosine 1 phosphate receptor 3, S1PR3, S1P3, S1P receptor Edg-3, S1P receptor EDG3, S1P receptor 3, LPB3, LPB 3, G protein coupled receptor endothelial differentiation gene 3, FLJ93220, FLJ37523, Endothelial differentiation sphingolipid G protein coupled receptor 3, Endothelial differentiation G-protein coupled receptor 3, Endothelial differentiation gene 3, EDG 3
Anti-EDG3 Polyclonal Antibody is a Rabbit antibody targeting EDG3. Anti-EDG3 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.
Anti-EDG3 Polyclonal Antibody
Anti-EDG3 Polyclonal Antibody
😃Good
Catalog No. TMAB-00589Alias Sphingosine 1 phosphate receptor Edg3, Sphingosine 1 phosphate receptor Edg 3, Sphingosine 1 phosphate receptor 3, S1PR3, S1P3, S1P receptor Edg-3, S1P receptor EDG3, S1P receptor 3, LPB3, LPB 3, G protein coupled receptor endothelial differentiation gene 3, FLJ93220, FLJ37523, Endothelial differentiation sphingolipid G protein coupled receptor 3, Endothelial differentiation G-protein coupled receptor 3, Endothelial differentiation gene 3, EDG 3
Anti-EDG3 Polyclonal Antibody is a Rabbit antibody targeting EDG3. Anti-EDG3 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $221 | 7-10 days | |
| 100 μL | $372 | 7-10 days | |
| 200 μL | $529 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-EDG3 Polyclonal Antibody is a Rabbit antibody targeting EDG3. Anti-EDG3 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM. |
| Alias | Sphingosine 1 phosphate receptor Edg3, Sphingosine 1 phosphate receptor Edg 3, Sphingosine 1 phosphate receptor 3, S1PR3, S1P3, S1P receptor Edg-3, S1P receptor EDG3, S1P receptor 3, LPB3, LPB 3, G protein coupled receptor endothelial differentiation gene 3, FLJ93220, FLJ37523, Endothelial differentiation sphingolipid G protein coupled receptor 3, Endothelial differentiation G-protein coupled receptor 3, Endothelial differentiation gene 3, EDG 3 |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat (predicted:Cow,Rabbit) |
| Verified Activity | 1. Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-EDG3 Polyclonal Antibody, Unconjugated (TMAB-00589) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. 2. Blank control: Hela. Primary Antibody (green line): Rabbit Anti-EDG3 antibody (TMAB-00589) Dilution: 0.2 μg/10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-PE Dilution: 1 μg/test. Protocol The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. 3. Sample: Lane1: Heart (Mouse) Lysate at 40 μg Primary: Anti-EDG3 (TMAB-00589) at 1/300 dilution Secondary: HRP conjugated Goat-Anti-rabbit IgG (secondary antibody) at 1/5000 dilution Predicted band size: 42 kDa Observed band size: 46 kDa 4. Sample: Lane 1: Cerebrum (Rat) Lysate at 40 μg Lane 2: Cerebrum (Mouse) Lysate at 40 μg Lane 3: Heart (Rat) Lysate at 40 μg Lane 4: Heart (Mouse) Lysate at 40 μg Primary: Anti-EDG3 (TMAB-00589) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 46 kDa Observed band size: 46 kDa |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 0.2u/test |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cell membrane; Multi-pass membrane protein. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | This gene encodes a member of the EDG family of receptors, which are G protein-coupled receptors. This protein has been identified as a functional receptor for sphingosine 1-phosphate and likely contributes to the regulation of angiogenesis and vascular endothelial cell function. [provided by RefSeq, Jul 2008]. |
| Immunogen | KLH conjugated synthetic peptide: human EDG3 |
| Antigen Species | Human |
| Gene ID | |
| Uniprot ID | |
| Biology Area | Endothelial Cell Markers,Vasoconstriction,GPCR |
| Molecular Weight | Theoretical: 42 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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