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Anti-EDG3 Polyclonal Antibody

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Catalog No. TMAB-00589
Alias Sphingosine 1 phosphate receptor Edg3, Sphingosine 1 phosphate receptor Edg 3, Sphingosine 1 phosphate receptor 3, S1PR3, S1P3, S1P receptor Edg-3, S1P receptor EDG3, S1P receptor 3, LPB3, LPB 3, G protein coupled receptor endothelial differentiation gene 3, FLJ93220, FLJ37523, Endothelial differentiation sphingolipid G protein coupled receptor 3, Endothelial differentiation G-protein coupled receptor 3, Endothelial differentiation gene 3, EDG 3

Anti-EDG3 Polyclonal Antibody is a Rabbit antibody targeting EDG3. Anti-EDG3 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.

Anti-EDG3 Polyclonal Antibody

Anti-EDG3 Polyclonal Antibody

😃Good
Catalog No. TMAB-00589Alias Sphingosine 1 phosphate receptor Edg3, Sphingosine 1 phosphate receptor Edg 3, Sphingosine 1 phosphate receptor 3, S1PR3, S1P3, S1P receptor Edg-3, S1P receptor EDG3, S1P receptor 3, LPB3, LPB 3, G protein coupled receptor endothelial differentiation gene 3, FLJ93220, FLJ37523, Endothelial differentiation sphingolipid G protein coupled receptor 3, Endothelial differentiation G-protein coupled receptor 3, Endothelial differentiation gene 3, EDG 3
Anti-EDG3 Polyclonal Antibody is a Rabbit antibody targeting EDG3. Anti-EDG3 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.
Pack SizePriceAvailabilityQuantity
50 μL$2217-10 days
100 μL$3727-10 days
200 μL$5297-10 days
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Product Introduction

Bioactivity
Description
Anti-EDG3 Polyclonal Antibody is a Rabbit antibody targeting EDG3. Anti-EDG3 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.
AliasSphingosine 1 phosphate receptor Edg3, Sphingosine 1 phosphate receptor Edg 3, Sphingosine 1 phosphate receptor 3, S1PR3, S1P3, S1P receptor Edg-3, S1P receptor EDG3, S1P receptor 3, LPB3, LPB 3, G protein coupled receptor endothelial differentiation gene 3, FLJ93220, FLJ37523, Endothelial differentiation sphingolipid G protein coupled receptor 3, Endothelial differentiation G-protein coupled receptor 3, Endothelial differentiation gene 3, EDG 3
Ig Type
IgG
Reactivity
Human,Mouse,Rat (predicted:Cow,Rabbit)
Verified Activity
1. Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-EDG3 Polyclonal Antibody, Unconjugated (TMAB-00589) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
2. Blank control: Hela.
Primary Antibody (green line): Rabbit Anti-EDG3 antibody (TMAB-00589)
Dilution: 0.2 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-PE
Dilution: 1 μg/test.
Protocol
The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.
3. Sample:
Lane1: Heart (Mouse) Lysate at 40 μg
Primary: Anti-EDG3 (TMAB-00589) at 1/300 dilution
Secondary: HRP conjugated Goat-Anti-rabbit IgG (secondary antibody) at 1/5000 dilution
Predicted band size: 42 kDa
Observed band size: 46 kDa
4. Sample:
Lane 1: Cerebrum (Rat) Lysate at 40 μg
Lane 2: Cerebrum (Mouse) Lysate at 40 μg
Lane 3: Heart (Rat) Lysate at 40 μg
Lane 4: Heart (Mouse) Lysate at 40 μg
Primary: Anti-EDG3 (TMAB-00589) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 46 kDa
Observed band size: 46 kDa
Application
WB,IHC-P,IHC-Fr,IF,FCM
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 0.2u/test
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationCell membrane; Multi-pass membrane protein.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundThis gene encodes a member of the EDG family of receptors, which are G protein-coupled receptors. This protein has been identified as a functional receptor for sphingosine 1-phosphate and likely contributes to the regulation of angiogenesis and vascular endothelial cell function. [provided by RefSeq, Jul 2008].
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human EDG3
Antigen Species
Human
Gene ID
Uniprot ID
Biology Area
Endothelial Cell Markers,Vasoconstriction,GPCR
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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