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Anti-EDA Polyclonal Antibody 2
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Catalog No. TMAB-05431
Anti-EDA Polyclonal Antibody 2 is a Rabbit antibody targeting EDA. Anti-EDA Polyclonal Antibody 2 can be used in WB,IHC-P,IHC-Fr,IF.
Anti-EDA Polyclonal Antibody 2
Anti-EDA Polyclonal Antibody 2
Copy Product Info😃Good
Catalog No. TMAB-05431
Anti-EDA Polyclonal Antibody 2 is a Rabbit antibody targeting EDA. Anti-EDA Polyclonal Antibody 2 can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $220 | 7-10 days | 7-10 days | |
| 100 μL | $371 | 7-10 days | 7-10 days | |
| 200 μL | $529 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction
Bioactivity
Antigen Details
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| Description | Anti-EDA Polyclonal Antibody 2 is a Rabbit antibody targeting EDA. Anti-EDA Polyclonal Antibody 2 can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat (predicted:Cow) |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (EDA) Polyclonal Antibody, Unconjugated (TMAB-05431) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. 2. Sample: Lane 1: Mouse Skin tissue lysates Lane 2: Mouse Stomach tissue lysates Lane 3: Mouse Kidney tissue lysates Lane 4: Rat Embryo tissue lysates Lane 5: Rat Skin tissue lysates Lane 6: Rat Stomach tissue lysates Lane 7: Rat Kidney tissue lysates Primary: Anti-EDA (TMAB-05431) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 41 kDa Observed band size: 41 kDa |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cell membrane; Single-pass type II membrane protein.Ectodysplasin-A, secreted form: Secreted. |
| Tissue Specificity | Not abundant; expressed in specific cell types of ectodermal (but not mesodermal) origin of keratinocytes, hair follicles, sweat glands. Also in adult heart, liver, muscle, pancreas, prostate, fetal liver, uterus, small intestine and umbilical chord. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | The protein encoded by the EDA gene is a type II membrane protein that can be cleaved by furin to produce a secreted form. The encoded protein, which belongs to the tumor necrosis factor family, acts as a homotrimer and may be involved in cell-cell signaling during the development of ectodermal organs. Defects in the EDA gene are a cause of ectodermal dysplasia, anhidrotic, (this is also known as X-linked hypohidrotic ectodermal dysplasia). Several transcript variants encoding many different isoforms have been found for this gene. AltName: ectodermal dysplasia protein; Ectodysplasin-A; EDA protein homolog; Tabby protein; Ectodysplasin-A, membrane form; secreted form EDA-A5. |
| Immunogen | KLH conjugated synthetic peptide: human Ectodysplasin-A, membrane form |
| Antigen Species | Human |
| Gene Name | EDA |
| Gene ID | |
| Protein Name | Ectodysplasin-A |
| Uniprot ID | |
| Function | Seems to be involved in epithelial-mesenchymal signaling during morphogenesis of ectodermal organs. Isoform 1 binds only to the receptor EDAR, while isoform 3 binds exclusively to the receptor XEDAR. |
| Molecular Weight | Theoretical: 41 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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