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Anti-ECM1 Polyclonal Antibody 2
Catalog No. TMAB-05425 Copy Product Info
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Anti-ECM1 Polyclonal Antibody 2 is a Rabbit antibody targeting ECM1. Anti-ECM1 Polyclonal Antibody 2 can be used in WB,IHC-P,IHC-Fr,IF.
Anti-ECM1 Polyclonal Antibody 2
Copy Product Info🥰Excellent
Catalog No. TMAB-05425
Anti-ECM1 Polyclonal Antibody 2 is a Rabbit antibody targeting ECM1. Anti-ECM1 Polyclonal Antibody 2 can be used in WB,IHC-P,IHC-Fr,IF.
Anti-ECM1 Polyclonal Antibody 2
| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 50 μL | $223 | 7-10 days | 7-10 days | |
| 100 μL | $372 | 7-10 days | 7-10 days | |
| 200 μL | $527 | 7-10 days | 7-10 days |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction
Bioactivity
Antigen Details
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| Description | Anti-ECM1 Polyclonal Antibody 2 is a Rabbit antibody targeting ECM1. Anti-ECM1 Polyclonal Antibody 2 can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| Reactivity | Mouse |
| Verified Activity | 1. Sample: Adrenal gland (Mouse) Lysate at 40 μg Primary: Anti-ECM1 at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 59 kD Observed band size: 59 kD 2. Sample: Bladder (Mouse) Lysate at 40 μg Primary: Anti-ECM1 (TMAB-05425) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 59 kD Observed band size: 59 kD 3. Paraformaldehyde-fixed, paraffin embedded (mouse kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (ECM1) Polyclonal Antibody, Unconjugated (TMAB-05425) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Secreted, extracellular space, extracellular matrix. |
| Tissue Specificity | Expressed in breast cancer tissues. Little or no expression observed in normal breast tissues. Expressed in skin; wide expression is observed throughout the dermis with minimal expression in the epidermis. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | Extracellular matrix protein 1 (ECM1) This family consists of several eukaryotic extracellular matrix protein 1 (ECM1) sequences. ECM1 has been shown to regulate endochondral bone formation, stimulate the proliferation of endothelial cells and induce angiogenesis. Mutations in the ECM1 gene can cause lipoid proteinosis, a disorder which causes generalised thickening of skin, mucosae and certain viscera. Classical features include beaded eyelid papules and laryngeal infiltration leading to hoarseness. |
| Immunogen | KLH conjugated synthetic peptide: mouse ECM1 |
| Antigen Species | Mouse |
| Gene Name | ECM1 |
| Gene ID | |
| Protein Name | Extracellular matrix protein 1 |
| Function | Involved in endochondral bone formation as negative regulator of bone mineralization. Stimulates the proliferation of endothelial cells and promotes angiogenesis. Inhibits MMP9 proteolytic activity. |
| Molecular Weight | Theoretical: 59 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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