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Anti-ECHS1 Antibody (4T819)

Catalog No. TMAB-05424 Copy Product Info
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Anti-ECHS1 Antibody (4T819) is a Mouse antibody targeting ECHS1. Anti-ECHS1 Antibody (4T819) can be used in WB,IHC-P,IHC-Fr,IF.

Anti-ECHS1 Antibody (4T819)

Copy Product Info
🥰Excellent
Catalog No. TMAB-05424

Anti-ECHS1 Antibody (4T819) is a Mouse antibody targeting ECHS1. Anti-ECHS1 Antibody (4T819) can be used in WB,IHC-P,IHC-Fr,IF.

Anti-ECHS1 Antibody
(4T819)
Pack SizePriceUSA StockGlobal StockQuantity
25 μL$1507-10 days7-10 days
50 μL$2637-10 days7-10 days
100 μL$4727-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Description
Anti-ECHS1 Antibody (4T819) is a Mouse antibody targeting ECHS1. Anti-ECHS1 Antibody (4T819) can be used in WB,IHC-P,IHC-Fr,IF.
Ig Type
IgG1, Kappa
Clone
4T819
Reactivity
Human
Verified Activity
1. Tissue: Human liver
Section type: Formalin fixed & Paraffin-embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:100
Primary ab incubation condition: 1 hour at room temperature
Counter stain: Hematoxylin
Comment: Color brown is the positive signal for TMAB-05424
2. Tissue: Human breast cancer
Section type: Formalin fixed & Paraffin-embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:100
Primary ab incubation condition: 1 hour at room temperature
Counter stain: Hematoxylin
Comment: Color brown is the positive signal for TMAB-05424
3. Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:1000
Primary ab incubation condition:4°C overnight
Lysate: HeLa, HepG2, 293, HT-29
Protein loading quantity: 20 μg
Exposure time: 30 s
Predicted MW: 31 kDa
Observed MW: 31 kDa
Application
Recommended Dose
WB: 1:500-2000; IHC-P: 1:50-100; IHC-Fr: 1:50-100; IF: 1:50-100
Antibody Type
Monoclonal
Host SpeciesMouse
Subcellular LocalizationMitochondrion matrix.
Tissue SpecificityLiver, fibroblast, muscle. Barely detectable in spleen and kidney.
ConstructionRecombinant Antibody
PurificationProtein G purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Research BackgroundECHS1 (Enoyl Coenzyme A hydratase, short chain, 1) catalyzes the hydration of 2-trans-enoyl-coenzyme A (CoA) intermediates to L-3-hydroxyacyl-CoAs, in the second step of the mitochondrial fatty acid beta-oxidation pathway.
Antigen Details
Gene Name
ECHS1
Gene ID
Protein Name
Enoyl-CoA hydratase, mitochondrial
Function
Straight-chain enoyl-CoA thioesters from C4 up to at least C16 are processed, although with decreasing catalytic rate.
Chemical Properties
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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