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Anti-ECE2 Polyclonal Antibody

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Catalog No. TMAB-05420 Copy Product Info
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Anti-ECE2 Polyclonal Antibody is a Rabbit antibody targeting ECE2. Anti-ECE2 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

Anti-ECE2 Polyclonal Antibody

Pack SizePriceUSA StockGlobal StockQuantity
50 µL$2217-10 days7-10 days
100 µL$3747-10 days7-10 days
200 µL$5297-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Description
Anti-ECE2 Polyclonal Antibody is a Rabbit antibody targeting ECE2. Anti-ECE2 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Ig Type
IgG
Reactivity
Human,Mouse,Rat
Verified Activity
1. Sample: 293T Cell (Human) Lysate at 40 μg
Primary: Anti-ECE2 (TMAB-05420) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 80-100 kD
Observed band size: 80 kD
2. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0); Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30 min; Antibody incubation with (ECE2) Polyclonal Antibody, Unconjugated (TMAB-05420) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP) and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0); Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30 min; Antibody incubation with (ECE2) Polyclonal Antibody, Unconjugated (TMAB-05420) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP) and DAB staining.
Application
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationGolgi apparatus membrane; Single-pass type II membrane protein (By similarity). Cytoplasmic granule membrane; Single-pass type II membrane protein (By similarity).
Tissue SpecificityExpressed in brain. Strongly down-regulated in inferior parietal lobe from Alzheimer disease patients (at protein level).
ConstructionPolyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Research BackgroundEndothelin-converting enzyme 2 (ECE2) converts big endothelin-1 to endothelin-1. Also involved in the processing of various neuroendocrine peptides, including neurotensin, angiotensin I, substance P, proenkephalin-derived peptides, and prodynorphin-derived peptides. May limit beta-amyloid peptide accumulation in brain. May also have methyltransferase activity. Expressed at high levels in central nervous system. Expressed in adrenal glands, ovary and uterus, and at low levels in heart.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human ECE2
Antigen Species
Human
Gene Name
ECE2
Gene ID
Protein Name
Endothelin-converting enzyme 2
Uniprot ID
Chemical Properties
Molecular WeightTheoretical: 80-100 kDa. Actual: 80 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
Related Tags: Anti-ECE2 Polyclonal Antibody molecular weight