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Anti-EB2 Polyclonal Antibody
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Catalog No. TMAB-05413
Anti-EB2 Polyclonal Antibody is a Rabbit antibody targeting EB2. Anti-EB2 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-EB2 Polyclonal Antibody
Anti-EB2 Polyclonal Antibody
Copy Product Info😃Good
Catalog No. TMAB-05413
Anti-EB2 Polyclonal Antibody is a Rabbit antibody targeting EB2. Anti-EB2 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $221 | 7-10 days | 7-10 days | |
| 100 μL | $373 | 7-10 days | 7-10 days | |
| 200 μL | $527 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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| Description | Anti-EB2 Polyclonal Antibody is a Rabbit antibody targeting EB2. Anti-EB2 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| Reactivity | Human,Mouse (predicted:Rat,Dog,Cow,Horse) |
| Verified Activity | 1. Sample: U937 (Human) Cell Lysate at 30 μg Primary: Anti-EB2 (TMAB-05413) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 36 kD Observed band size: 35 kD 2. Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (EB2) Polyclonal Antibody, Unconjugated (TMAB-05413) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cytoplasm, cytoskeleton. |
| Tissue Specificity | Expressed in different tumor cell lines. Up-regulated in activated B- and T-lymphocytes. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | The protein encoded by this gene shares significant homology to the adenomatous polyposis coli (APC) protein-binding EB1 gene family. This protein is a microtubule-associated protein that is necessary for spindle symmetry during mitosis. It is thought to play a role in the tumorigenesis of colorectal cancers and the proliferative control of normal cells. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jan 2012]. |
| Immunogen | KLH conjugated synthetic peptide: human End binding protein 2 |
| Antigen Species | Human |
| Gene Name | MAPRE2 |
| Gene ID | |
| Protein Name | Microtubule-associated protein RP/EB family member 2 |
| Uniprot ID | |
| Function | May be involved in microtubule polymerization, and spindle function by stabilizing microtubules and anchoring them at centrosomes. May play a role in cell migration. |
| Molecular Weight | Theoretical: 36 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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