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Anti-E-Cadherin/Cadherin-1 Polyclonal Antibody
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Catalog No. TMAB-00584
Anti-E-Cadherin/Cadherin-1 Polyclonal Antibody is a Rabbit antibody targeting E-Cadherin/Cadherin-1. Anti-E-Cadherin/Cadherin-1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-E-Cadherin/Cadherin-1 Polyclonal Antibody
Anti-E-Cadherin/Cadherin-1 Polyclonal Antibody
😃Good
Catalog No. TMAB-00584
Anti-E-Cadherin/Cadherin-1 Polyclonal Antibody is a Rabbit antibody targeting E-Cadherin/Cadherin-1. Anti-E-Cadherin/Cadherin-1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $222 | 7-10 days | |
| 100 μL | $374 | 7-10 days | |
| 200 μL | $529 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Antibody Type: Rabbit Polyclonal Application: WB,IHC-P,IHC-Fr,IF Reactivity: Human,Rat (predicted:Rabbit,Horse,Cow,Pig,Mouse) |
| Ig Type | IgG |
| Reactivity | Human,Rat (predicted:Rabbit,Horse,Cow,Pig,Mouse) |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (Human colon carcinoma); Antigen retrieval by microwave in sodium citrate buffer (pH6.0); Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30 min; Antibody incubation with (E cadherin) Polyclonal Antibody, Unconjugated (TMAB-00584) at 1:400 overnight at 4°C, followed by conjugation to the secondary antibody (labeled with HRP) and DAB staining. 2. Sample: HT29 (Human) Cell Lysate at 30 μg PC-3 (Human) Cell Lysate at 30 μg Primary: Anti-E cadherin (TMAB-00584) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 20/80 kDa Observed band size: 130 kDa 3. Paraformaldehyde-fixed, paraffin embedded (Human stomach); Antigen retrieval by microwave in sodium citrate buffer (pH6.0); Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30 min; Antibody incubation with (E cadherin) Polyclonal Antibody, Unconjugated (TMAB-00584) at 1:400 overnight at 4°C, followed by conjugation to the secondary antibody (labeled with HRP) and DAB staining. 4. Sample: Lane 1: A431 (Human) Cell Lysate at 30 μg Lane 2: A549 (Human) Cell Lysate at 30 μg Lane 3: HepG2 (Human) Cell Lysate at 30 μg Primary: Anti-E cadherin (TMAB-00584) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 125 kDa Observed band size: 125 kDa 5. Paraformaldehyde-fixed, paraffin embedded (rat spinal cord); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (E cadherin) Polyclonal Antibody, Unconjugated (TMAB-00584) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 6. Paraformaldehyde-fixed, paraffin embedded (rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (E cadherin) Polyclonal Antibody, Unconjugated (TMAB-00584) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 7. Paraformaldehyde-fixed, paraffin embedded (human colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (E cadherin) Polyclonal Antibody, Unconjugated (TMAB-00584) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
| Application | WB,IHC-P,IHC-Fr,IF |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cell junction. Cell membrane; Single-pass type I membrane protein. |
| Construction | Hybridoma Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | This gene encodes a classical cadherin of the cadherin superfamily. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature glycoprotein. This calcium-dependent cell-cell adhesion protein is comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function of this gene is thought to contribute to cancer progression by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. This gene is present in a gene cluster with other members of the cadherin family on chromosome 16. [provided by RefSeq, Nov 2015] |
| Immunogen | KLH conjugated synthetic peptide: human E-cadherin |
| Antigen Species | Human |
| Gene ID | 999 |
| Uniprot ID | |
| Biology Area | Cadherins,Kidney development,e cadherin,Cadherins,Calcium Binding Proteins |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
Sci Citations
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