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Anti-E-Cadherin/Cadherin-1 Antibody (2V272)

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Catalog No. TMAY-00464
Alias UVO, LCAM, E-Cadherin, E-cad, ECAD, CDHE, CDH1, CD324, cadherin 1, type 1, E-cadherin (epithelial), Arc-1

Anti-E-Cadherin/Cadherin-1 Antibody (2V272) is a Rabbit antibody targeting E-Cadherin/Cadherin-1. Anti-E-Cadherin/Cadherin-1 Antibody (2V272) can be used in WB,IP.

Anti-E-Cadherin/Cadherin-1 Antibody (2V272)

Anti-E-Cadherin/Cadherin-1 Antibody (2V272)

😃Good
Catalog No. TMAY-00464Alias UVO, LCAM, E-Cadherin, E-cad, ECAD, CDHE, CDH1, CD324, cadherin 1, type 1, E-cadherin (epithelial), Arc-1
Anti-E-Cadherin/Cadherin-1 Antibody (2V272) is a Rabbit antibody targeting E-Cadherin/Cadherin-1. Anti-E-Cadherin/Cadherin-1 Antibody (2V272) can be used in WB,IP.
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100 μL$269 7-10 days
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Product Introduction

Bioactivity
Description
Anti-E-Cadherin/Cadherin-1 Antibody (2V272) is a Rabbit antibody targeting E-Cadherin/Cadherin-1. Anti-E-Cadherin/Cadherin-1 Antibody (2V272) can be used in WB,IP.
AliasUVO, LCAM, E-Cadherin, E-cad, ECAD, CDHE, CDH1, CD324, cadherin 1, type 1, E-cadherin (epithelial), Arc-1
Ig Type
Monoclonal Rabbit IgG
Clone
2V272
Reactivity
Human
Verified Activity
1. E-cad was immunoprecipitated using:

-Lane A:0.5 mg MCF-7 Whole Cell Lysate.

-Lane B:0.5 mg A431 Whole Cell Lysate.

-4 µL anti-E-cad rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose.

-Primary antibody:

-Anti-E-cad rabbit monoclonal antibody, at 1:100 dilution.

-Secondary antibody:

-Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution.

-Developed using the odyssey technique.

-Performed under reducing conditions.

-Predicted band size: 97 kDa.

-Observed band size: 97 kDa.

2. Anti-E-cad rabbit monoclonal antibody at 1:500 dilution.

-Lane A: MCF7 Whole Cell Lysate.

-Lane B: A431 Whole Cell lysate.

-Lysates/proteins at 30 μg per lane.

-Secondary

-Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

-Developed using the Odyssey technique.

-Performed under reducing conditions.

-Predicted band size:97 kDa.

-Observed band size:130 kDa
Application
WB,IP
Recommended Dose
WB: 1:500-1:2000; IP: 4-8 μL/mg of lysate
Antibody Type
Monoclonal
Host SpeciesRabbit
ConstructionThis antibody was obtained from a rabbit immunized with purified, recombinant Human E-Cadherin / CDH1 / E-cad / CD324 (rh E-Cadherin / CDH1 / E-cad / CD324; NP_004351.1Met1-Ile707).
PurificationProtein A
AppearanceLiquid
Formulation0.2 μm filtered solution in PBS
Research BackgroundCadherins are calcium-dependent cell adhesion proteins which preferentially interact with themselves in a homophilic manner in connecting cells, and thus may contribute to the sorting of heterogeneous cell type. E-cadherin (E-Cad), also known as CDH1 and CD324, is a calcium-dependent cell adhesion molecule the intact function of which is crucial for the establishment and maintenance of epithelial tissue polarity and structural integrity. Mutations in CDH1 occur in diffuse type gastric cancer, lobular breast cancer, and endometrial cancer. In human cancers, partial or complete loss of E-cadherin expression correlates with malignancy. During apoptosis or with calcium influx, E-Cad is cleaved by the metalloproteinase to produce fragments of about 38 kDa (E-CAD/CTF1), 33 kDa (E-CAD/CTF2) and 29 kDa (E-CAD/CTF3), respectively. E-Cad has been identified as a potent invasive suppressor, as downregulation of E-cadherin expression is involved in dysfunction of the cell-cell adhesion system, and often correlates with strong invasive potential and poor prognosis of human carcinomas.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
Recombinant Human E-Cadherin / CDH1 / E-cad / CD324 Protein
Antigen Species
Human
Biology Area
Hemangioblast Markers, Tumor Suppressors
Chemical Properties
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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