Anti-DZIP3 Polyclonal Antibody is a Rabbit antibody targeting DZIP3. Anti-DZIP3 Polyclonal Antibody can be used in FCM.

IgG

Mouse (predicted:Human,Rat,Dog,Cow,Horse,Rabbit)

Blank control: Mouse kidney.
Primary Antibody (green line): Rabbit Anti-DZIP3 antibody (TMAB-05377)
Dilution: 2 μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-AF488
Dilution: 1 μg /test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

FCM: 2μg/Test

Polyclonal

KLH conjugated synthetic peptide: Human DZIP3

Human

DZIP3

E3 ubiquitin-protein ligase DZIP3

E3 Ubiquitin ligase proteins mediate ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Able to specifically bind RNA.