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Anti-DYKDDDDK-Tag Polyclonal Antibody
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Catalog No. TMAC-01194
Anti-DYKDDDDK-Tag Polyclonal Antibody is a Rabbit antibody targeting DYKDDDDK-Tag. Anti-DYKDDDDK-Tag Polyclonal Antibody can be used in WB.
Anti-DYKDDDDK-Tag Polyclonal Antibody
Anti-DYKDDDDK-Tag Polyclonal Antibody
😃Good
Catalog No. TMAC-01194
Anti-DYKDDDDK-Tag Polyclonal Antibody is a Rabbit antibody targeting DYKDDDDK-Tag. Anti-DYKDDDDK-Tag Polyclonal Antibody can be used in WB.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $217 | 7-10 days | |
| 100 μL | $317 | 7-10 days |
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Antigen Details
Chemical Properties
| Description | Anti-DYKDDDDK-Tag Polyclonal Antibody is a Rabbit antibody targeting DYKDDDDK-Tag. Anti-DYKDDDDK-Tag Polyclonal Antibody can be used in WB. |
| Ig Type | IgG |
| Verified Activity | 1. Western Blot analysis using FLAG-tag Polyclonal Antibody against HEK293 cells transfected with vector overexpressing FLAG tag (1) and untransfected (2). Secondary antibody was diluted at 1:20000. |
| Application | |
| Recommended Dose | WB: 1:1000-3000 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Construction | Polyclonal Antibody |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Appearance | Liquid |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research Background | The DYKDDDDK (FLAG) peptide has been used extensively as a general tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion. N-terminal FLAG vectors provide an Ek cleavage site for removal of the fusion tag. The FLAG peptide is likely to be located on the surface of a fusion protein because of its hydrophilic nature. As a result, the FLAG peptide is more likely to be accessible to antibodies. A FLAG-tag can be used in many different assays that require recognition by an antibody, such as western blotting, immunocytochemistry, immunoprecipitation, flow cytometry, protein purification, and in the study of protein-protein interactions, cell ultrastructure, and protein localization and so on. |
| Conjucates | Unconjugated |
| Immunogen | KLH conjugated synthetic peptide: DDDDK |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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