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Anti-DYKDDDDK-Tag Polyclonal Antibody

Catalog No. TMAC-01194 Copy Product Info
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Anti-DYKDDDDK-Tag Polyclonal Antibody is a Rabbit antibody targeting DYKDDDDK-Tag. Anti-DYKDDDDK-Tag Polyclonal Antibody can be used in WB.

Anti-DYKDDDDK-Tag Polyclonal Antibody

Copy Product Info
🥰Excellent
Catalog No. TMAC-01194

Anti-DYKDDDDK-Tag Polyclonal Antibody is a Rabbit antibody targeting DYKDDDDK-Tag. Anti-DYKDDDDK-Tag Polyclonal Antibody can be used in WB.

Anti-DYKDDDDK-Tag Polyclonal Antibody
Pack SizePriceUSA StockGlobal StockQuantity
50 μL$2177-10 days7-10 days
100 μL$3177-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Description
Anti-DYKDDDDK-Tag Polyclonal Antibody is a Rabbit antibody targeting DYKDDDDK-Tag. Anti-DYKDDDDK-Tag Polyclonal Antibody can be used in WB.
Ig Type
IgG
Verified Activity
1. Western Blot analysis using FLAG-tag Polyclonal Antibody against HEK293 cells transfected with vector overexpressing FLAG tag (1) and untransfected (2). Secondary antibody was diluted at 1:20000.
verifiedActivity
Application
Recommended Dose
WB: 1:1000-3000
Antibody Type
Polyclonal
Host SpeciesRabbit
ConstructionPolyclonal Antibody
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
AppearanceLiquid
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Research BackgroundThe DYKDDDDK (FLAG) peptide has been used extensively as a general tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion. N-terminal FLAG vectors provide an Ek cleavage site for removal of the fusion tag. The FLAG peptide is likely to be located on the surface of a fusion protein because of its hydrophilic nature. As a result, the FLAG peptide is more likely to be accessible to antibodies. A FLAG-tag can be used in many different assays that require recognition by an antibody, such as western blotting, immunocytochemistry, immunoprecipitation, flow cytometry, protein purification, and in the study of protein-protein interactions, cell ultrastructure, and protein localization and so on.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
KLH conjugated synthetic peptide: DDDDK
Chemical Properties
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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