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Anti-Flag Tag Antibody (5I361)
😃Good
Catalog No. TMAY-00094
Anti-Flag Tag Antibody (5I361) is a Mouse antibody targeting Flag Tag. Anti-Flag Tag Antibody (5I361) can be used in ICC/IF.
Anti-Flag Tag Antibody (5I361)
Anti-Flag Tag Antibody (5I361)
😃Good
Catalog No. TMAY-00094
Anti-Flag Tag Antibody (5I361) is a Mouse antibody targeting Flag Tag. Anti-Flag Tag Antibody (5I361) can be used in ICC/IF.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 20 μL | $92 | 7-10 days | 7-10 days | |
| 100 μL | $186 | 7-10 days | 7-10 days | |
| 500 μL | $469 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Anti-Flag Tag Antibody (5I361) is a Mouse antibody targeting Flag Tag. Anti-Flag Tag Antibody (5I361) can be used in ICC/IF. |
| Ig Type | Mouse IgG2b |
| Clone | 5I361 |
| Specificity | Recognize N-terminal and C-terminal Flag Tag in fusion proteins |
| Verified Activity | Immunofluorescence staining of flag-Tag in 293 cells, transfected with PCMV-CDH1-flag (Figure A), pSTEP2-Flag-FABP4-GST (Figure B), pSTEP2-Flag-ARG1-GST (Figure C), Flag-PRMT5-His (Figure D),Flag-PRMT6-His (Figure E) and His-flag-CD38(Figure F). Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-flag-Tag monoclonal antibody at 37°C 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI (blue). |
| Application | |
| Recommended Dose | ICC-IF: 1:50-1:1000 |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Construction | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, the synthetic peptide corresponding to the DYKDDDDK-tag sequence (CDYKDDDDK). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Conjucates | Unconjugated |
| Others Formats | HRP |
| Antibody Types Available | 2 |
| Immunogen | A synthetic peptide: flag-tag sequence (DYKDDDDK). |
| Antigen Species | other |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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