Anti-dUTPase Polyclonal Antibody is a Rabbit antibody targeting dUTPase. Anti-dUTPase Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.

IgG

Human (predicted:Mouse,Rat,Dog,Pig,Cow,Rabbit)

1. Tissue/cell: human brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-dUTPase Polyclonal Antibody, Unconjugated (TMAB-05355) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
2. Paraformaldehyde-fixed, paraffin embedded (human tonsil); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (dUTPase) Polyclonal Antibody, Unconjugated (TMAB-05355) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.

IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human dUTPase

Human

DUT

Deoxyuridine 5'-triphosphate nucleotidohydrolase, mitochondrial

This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA.