Anti-DUSP4 Polyclonal Antibody is a Rabbit antibody targeting DUSP4. Anti-DUSP4 Polyclonal Antibody can be used in WB,FCM.

IgG

Human,Mouse (predicted:Rat,Chicken,Dog,Cow,Rabbit)

1. Blank control: A431.
Primary Antibody (green line): Rabbit Anti-DUSP4 antibody (TMAB-05346)
Dilution: 1 μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-AF647
Dilution: 1 μg /test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
2. Sample:
RAW264.7 (Mouse) Cell Lysate at 30 μg
Primary: Anti-DUSP4 (TMAB-05346) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 44 kD
Observed band size: 44 kD

WB: 1:500-2000; FCM: 1μg/Test

Polyclonal

KLH conjugated synthetic peptide: human MKP-2

Human

DUSP4

Dual specificity protein phosphatase 4