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Anti-DSPP Polyclonal Antibody 2
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Catalog No. TMAB-00574
Anti-DSPP Polyclonal Antibody 2 is a Rabbit antibody targeting DSPP. Anti-DSPP Polyclonal Antibody 2 can be used in WB,IHC-P,IHC-Fr,IF.
Anti-DSPP Polyclonal Antibody 2
Anti-DSPP Polyclonal Antibody 2
😃Good
Catalog No. TMAB-00574
Anti-DSPP Polyclonal Antibody 2 is a Rabbit antibody targeting DSPP. Anti-DSPP Polyclonal Antibody 2 can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $221 | 7-10 days | |
| 100 μL | $374 | 7-10 days | |
| 200 μL | $529 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Antibody Type: Rabbit Polyclonal Application: WB,IHC-P,IHC-Fr,IF Reactivity: Human |
| Ig Type | IgG |
| Reactivity | Human |
| Verified Activity | 1. Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-DSPP Polyclonal Antibody, Unconjugated (TMAB-00574) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. 2. Sample: Lane 1: Hela (Human) Cell Lysate at 30 μg Lane 2: SW480 (Human) Cell Lysate at 30 μg Lane 3: Du145 (Human) Cell Lysate at 30 μg Primary: Anti-DSPP (TMAB-00574) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 140 kDa Observed band size: 130 kDa 3. Sample: A549 (Human) Cell Lysate at 30 μg Primary: Anti-DSPP (TMAB-00574) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 47/129 kDa Observed band size: 129 kDa |
| Application | WB,IHC-P,IHC-Fr,IF |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Secreted, extracellular space, extracellular matrix. |
| Construction | Hybridoma Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | This gene encodes two principal proteins of the dentin extracellular matrix of the tooth. The preproprotein is secreted by odontoblasts and cleaved into dentin sialoprotein and dentin phosphoprotein. Dentin phosphoprotein is thought to be involved in the biomineralization process of dentin. Mutations in this gene have been associated with dentinogenesis imperfecta-1; in some individuals, dentinogenesis imperfecta occurs in combination with an autosomal dominant form of deafness. Allelic differences due to repeat polymorphisms have been found for this gene. [provided by RefSeq, Jul 2008] |
| Immunogen | KLH conjugated synthetic peptide: human Dentin sialoprotein |
| Antigen Species | Human |
| Gene ID | 1834 |
| Uniprot ID | |
| Biology Area | Cell differentiation,Bone,Other ECM Proteins,Bone,Calcium Binding Proteins |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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