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Anti-DSPP Polyclonal Antibody

😃Good
Catalog No. TMAB-00573

Anti-DSPP Polyclonal Antibody is a Rabbit antibody targeting DSPP. Anti-DSPP Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

Anti-DSPP Polyclonal Antibody

Anti-DSPP Polyclonal Antibody

😃Good
Catalog No. TMAB-00573
Anti-DSPP Polyclonal Antibody is a Rabbit antibody targeting DSPP. Anti-DSPP Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Pack SizePriceAvailabilityQuantity
50 μL$222 7-10 days
100 μL$373 7-10 days
200 μL$529 7-10 days
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Product Introduction

Bioactivity
Description
Anti-DSPP Polyclonal Antibody is a Rabbit antibody targeting DSPP. Anti-DSPP Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Ig Type
IgG
Reactivity
Human
Verified Activity
1. Sample:
Lane 1: Hela (Human) Cell Lysate at 30 μg
Lane 2: SW480 (Human) Cell Lysate at 30 μg
Lane 3: Du145 (Human) Cell Lysate at 30 μg
Primary: Anti-DSPP (TMAB-00573) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 140 kDa
Observed band size: 130 kDa
2. Sample: LOVO (Human) Cell Lysate at 30 μg 293t (Human) Cell Lysate at 30 μg
Primary: Anti-DSPP (TMAB-00573) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size:140 kDa
Observed band size: 130 kDa
3. Tissue/cell: human laryngocarcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-DSPP Polyclonal Antibody, Unconjugated (TMAB-00573) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
4. Sample:
EC-9706 (Human) Cell Lysate at 30 μg
MCF-7 (Human) Cell Lysate at 30 μg
Primary: Anti-DSPP (TMAB-00573) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 47/129 kDa
Observed band size: 129 kDa
Application
WB,IHC-P,IHC-Fr,IF
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationSecreted, extracellular space, extracellular matrix.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundThis gene encodes two principal proteins of the dentin extracellular matrix of the tooth. The preproprotein is secreted by odontoblasts and cleaved into dentin sialoprotein and dentin phosphoprotein. Dentin phosphoprotein is thought to be involved in the biomineralization process of dentin. Mutations in this gene have been associated with dentinogenesis imperfecta-1; in some individuals, dentinogenesis imperfecta occurs in combination with an autosomal dominant form of deafness. Allelic differences due to repeat polymorphisms have been found for this gene. [provided by RefSeq, Jul 2008]
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human Dentin sialoprotein
Antigen Species
Human
Gene ID
Uniprot ID
Biology Area
Cell differentiation,Bone,Other ECM Proteins,Bone,Calcium Binding Proteins
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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