Anti-DRD1 Polyclonal Antibody 2 is a Rabbit antibody targeting DRD1. Anti-DRD1 Polyclonal Antibody 2 can be used in WB,IHC-P,IHC-Fr,IF,FCM.

IgG

Human,Rat

1. Cell: SH-SY5Y
Concentration: 1:100
Host/Isotype: Rabbit/IgG
Flow cytometric analysis of Rabbit IgG isotype control (Cat#: TMAB-05315) on SH-SY5Y (green) compared with control in the absence of primary antibody (blue) followed by Alexa Fluor 488-conjugated goat anti-rabbit Igg (H+L) secondary antibody.
2. Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-Dopamine Receptor D1 Polyclonal Antibody, Unconjugated (TMAB-05315) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
3. Sample:
A431 Cell (Human) Lysate at 40 μg
HepG2 Cell (Human) Lysate at 40 μg
Primary: Anti-Dopamine Receptor D1 (TMAB-05315) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 50 kD
Observed band size: 60 kD
4. Sample: A549 Cell (Human) Lysate at 40 μg
Primary: Anti-Dopamine Receptor D1 (TMAB-05315) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 50 kD
Observed band size: 60 kD

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 1μg/Test

Polyclonal

KLH conjugated synthetic peptide: human Dopamine Receptor D1

Human

DRD1

D(1A) dopamine receptor

Dopamine receptor whose activity is mediated by G proteins which activate adenylyl cyclase.