Anti-DRAM Polyclonal Antibody is a Rabbit antibody targeting DRAM. Anti-DRAM Polyclonal Antibody can be used in ICC/IF,IF,IHC-Fr,IHC-P.

IgG

Human,Rat (predicted:Mouse,Dog,Pig,Horse,Rabbit,Sheep)

1. Tissue/cell: human skin squamous carcinoma;
Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-DRAM Polyclonal Antibody, Unconjugated (TMAB-00569) 1:100, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
2. Tissue/cell: rat colon tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-DRAM Polyclonal Antibody, Unconjugated (TMAB-00569) 1:100, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.

IHC-P: 1:100-500; IHC-Fr: 1:100-500; ICC/IF: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human DRAM

Human

DRAM1

DNA damage-regulated autophagy modulator protein 1

p53 pathway,Signal Transduction,Autophagy,Autophagy,p53 Pathway,Autophagy and mitophagy

Lysosomal modulator of autophagy that plays a central role in p53/TP53-mediated apoptosis. Not involved in p73/TP73-mediated autophagy.