Anti-DP1 Polyclonal Antibody is a Rabbit antibody targeting DP1. Anti-DP1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Human,Mouse,Rat (predicted:Chicken,Dog,Cow,Horse,Rabbit)

1. Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-DP1/TFDP1 Polyclonal Antibody, Unconjugated (TMAB-05294) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
2. Sample:
Lane 1: Mouse Testis tissue lysates
Lane 2: Rat Ovary tissue lysates
Lane 3: Human A431 cell lysates
Lane 4: Human Jurkat cell lysates
Primary: Anti-DP1 (TMAB-05294) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 45 kD
Observed band size: 45 kD

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human TFDP1

Human

TFDP1

Transcription factor Dp-1

The DP2/E2F complex functions in the control of cell-cycle progression from G1 to S phase. The E2F-1/DP complex appears to mediate both cell proliferation and apoptosis.