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Anti-Doublecortin/DCX Antibody (1Q732)
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Catalog No. TMAY-02589
Alias XLIS, SCLH, LISX, doublecortin, DC, DBCN
Anti-Doublecortin/DCX Antibody (1Q732) is a Mouse antibody targeting Doublecortin/DCX. Anti-Doublecortin/DCX Antibody (1Q732) can be used in ICC/IF.
Anti-Doublecortin/DCX Antibody (1Q732)
Anti-Doublecortin/DCX Antibody (1Q732)
😃Good
Catalog No. TMAY-02589Alias XLIS, SCLH, LISX, doublecortin, DC, DBCN
Anti-Doublecortin/DCX Antibody (1Q732) is a Mouse antibody targeting Doublecortin/DCX. Anti-Doublecortin/DCX Antibody (1Q732) can be used in ICC/IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 100 μL | $198 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Anti-Doublecortin/DCX Antibody (1Q732) is a Mouse antibody targeting Doublecortin/DCX. Anti-Doublecortin/DCX Antibody (1Q732) can be used in ICC/IF. |
| Alias | XLIS, SCLH, LISX, doublecortin, DC, DBCN |
| Ig Type | Monoclonal Mouse IgG2a |
| Clone | 1Q732 |
| Reactivity | Human |
| Verified Activity | Immunofluorescence staining of Human DCX in HepG2 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Human DCX monoclonal antibody (1:60) at 37°C 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm. |
| Application | ICC/IF |
| Recommended Dose | ICC-IF: 1:20-1:100 |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Construction | This product is a recombinant monoclonal antibody expressed from HEK293 cells. |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS with 10% Trehalose, 0.02% Tween 80, pH7.0 |
| Research Background | DCX (doublecortin, N-GST chimera)contains 2 doublecortin domains and belongs to the doublecortin family. It is highly expressed in neuronal cells of fetal brain, but not expressed in other fetal tissues. In the adult, it is highly expressed in the brain frontal lobe, but very low expression in other regions of brain, and not detected in heart, placenta, lung, liver, skeletal muscles, kidney and pancreas. DCX is a microtubule-associated protein required for initial steps of neuronal dispersion and cortex lamination during cerebral cortex development. It may act by competing with the putative neuronal protein kinase DCAMKL1 in binding to a target protein. DCX may in that way participate in a signaling pathway that is crucial for neuronal interaction before and during migration, possibly as part of a calcium ion-dependent signal transduction pathway. It may be part with LIS-1 of a overlapping, but distinct, signaling pathways that promote neuronal migration. Defects in DCX are the cause of lissencephaly X-linked type 1 and subcortical band heterotopia X-linked. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Human Doublecortin/DCX Protein (TMPY-02718) |
| Antigen Species | Human |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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