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Anti-DOLPP1 Polyclonal Antibody

Catalog No. TMAB-05283 Copy Product Info
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Anti-DOLPP1 Polyclonal Antibody is a Rabbit antibody targeting DOLPP1. Anti-DOLPP1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

Anti-DOLPP1 Polyclonal Antibody

Copy Product Info
🥰Excellent
Catalog No. TMAB-05283

Anti-DOLPP1 Polyclonal Antibody is a Rabbit antibody targeting DOLPP1. Anti-DOLPP1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

Anti-DOLPP1 Polyclonal Antibody
Pack SizePriceUSA StockGlobal StockQuantity
50 μL$2207-10 days7-10 days
100 μL$3727-10 days7-10 days
200 μL$5297-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Description
Anti-DOLPP1 Polyclonal Antibody is a Rabbit antibody targeting DOLPP1. Anti-DOLPP1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Ig Type
IgG
Reactivity
Mouse,Rat (predicted:Human,Chicken,Dog,Pig,Cow,Horse,Sheep)
Verified Activity
1. Sample: Cerebrum (Mouse) Lysate at 40 μg
Primary: Anti-DOLPP1 (TMAB-05283) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 27 kD
Observed band size: 27 kD
2. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (DOLPP1) Polyclonal Antibody, Unconjugated (TMAB-05283) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
Application
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationEndoplasmic reticulum membrane; Multi-pass membrane protein (By similarity).
ConstructionPolyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Research BackgroundA similar gene has been characterized in mice and encodes dolichyl pyrophosphate (Dol-P-P) phosphatase. This protein dephosphorylates dolichyl pyrophosphate so that it may be re-utilized as a glycosyl carrier lipid by the oligosaccharyltransferase multisubunit complex in the ER. Alternative splicing results in multiple transcript variants and protein isoforms. [provided by RefSeq, Jun 2012]
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human DOLPP1
Antigen Species
Human
Gene Name
DOLPP1
Gene ID
Protein Name
Dolichyldiphosphatase 1
Uniprot ID
Function
DOLPP1 is a multi-pass membrane protein(By similarity). It belongs to the dolichyldiphosphatase family. It is required for efficient N-glycosylation and is necessary for maintaining optimal levels of dolichol-linked oligosaccharides. DOLPP1 hydrolyzes dolichyl pyrophosphate at a very high rate and dolichyl monophosphate at a much lower rate. It does not act on phosphatidate
Chemical Properties
Molecular WeightTheoretical: 27 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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