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Anti-DNASE1L1 Polyclonal Antibody
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Catalog No. TMAB-05251
Anti-DNASE1L1 Polyclonal Antibody is a Rabbit antibody targeting DNASE1L1. Anti-DNASE1L1 Polyclonal Antibody can be used in FCM.
Anti-DNASE1L1 Polyclonal Antibody
Anti-DNASE1L1 Polyclonal Antibody
Copy Product Info😃Good
Catalog No. TMAB-05251
Anti-DNASE1L1 Polyclonal Antibody is a Rabbit antibody targeting DNASE1L1. Anti-DNASE1L1 Polyclonal Antibody can be used in FCM.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $220 | 7-10 days | 7-10 days | |
| 100 μL | $372 | 7-10 days | 7-10 days | |
| 200 μL | $527 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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| Description | Anti-DNASE1L1 Polyclonal Antibody is a Rabbit antibody targeting DNASE1L1. Anti-DNASE1L1 Polyclonal Antibody can be used in FCM. |
| Ig Type | IgG |
| Reactivity | Mouse (predicted:Human,Rat,Dog,Horse) |
| Verified Activity | Blank control: Mouse kidney. Primary Antibody (green line): Rabbit Anti-DNASE1L1 antibody (TMAB-05251) Dilution: 2 μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF488 Dilution: 1 μg /test. Protocol The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed. |
| Application | |
| Recommended Dose | FCM: 2μg/Test |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Endoplasmic reticulum. |
| Tissue Specificity | Highest levels in skeletal and cardiac muscles. Detectable in all other tissues tested except brain. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | DNASE1L1 is a 302 amino acid protein that localizes to the endoplasmic reticulum and belongs to the deoxyribonuclease family. Expressed at high levels in cardiac and skeletal muscle and at lower levels in a variety of tissues throughout the body, DNASE1L1 exists as multiple alternatively spliced isoforms and is thought to function in a similar manner to DNase l, possibly mediating internucleosomal DNA degradation via catalytic cleavage events. The gene encoding DNASE1L1 maps to human chromosome X, which contains nearly 153 million base pairs and houses over 1,000 genes. In conjunction with chromosome Y, chromosome X is responsible for sex determination. There are a number of conditions related to an abnormal number and combination of sex chromosomes, some of which include Turner's syndrome, color blindness, hemophilia and Duchenne muscular dystrophy. |
| Immunogen | KLH conjugated synthetic peptide: human DNASE1L1 |
| Antigen Species | Human |
| Gene Name | DNASE1L1 |
| Gene ID | |
| Protein Name | Deoxyribonuclease-1-like 1 |
| Uniprot ID |
| Molecular Weight | Theoretical: 32 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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