Anti-DNase II Polyclonal Antibody 2 is a Rabbit antibody targeting DNase II. Anti-DNase II Polyclonal Antibody 2 can be used in WB.

IgG

Human,Mouse,Rat (predicted:Dog,Pig,Cow,Horse,Rabbit,Sheep)

1. Paraformaldehyde-fixed, paraffin embedded (Mouse cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (DNase II) Polyclonal Antibody, Unconjugated (TMAB-05249) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
2. Sample:
Lane 1: Cerebrum (Mouse) Lysate at 40 μg
Lane 2: Stomach (Mouse) Lysate at 40 μg
Lane 3: Liver (Mouse) Lysate at 40 μg
Lane 4: Kidney (Mouse) Lysate at 40 μg
Lane 5: Testis (Mouse) Lysate at 40 μg
Lane 6: Cerebrum (Rat) Lysate at 40 μg
Lane 7: Liver (Rat) Lysate at 40 μg
Lane 8: Kidney (Rat) Lysate at 40 μg
Lane 9: A431 (Human) Cell Lysate at 30 μg
Lane 10: Huvec (Human) Cell Lysate at 30 μg
Primary: Anti-DNase II (TMAB-05249) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 38 kD
Observed band size: 45 kD

WB: 1:500-2000

Polyclonal

KLH conjugated synthetic peptide: human DNase II

Human

DNASE2

DNASE1

Hydrolyzes DNA under acidic conditions with a preference for double-stranded DNA. Plays a major role in the degradation of nuclear DNA in cellular apoptosis during development. Necessary for proper fetal development and for definitive erythropoiesis in fetal liver, where it degrades nuclear DNA expelled from erythroid precursor cells.