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Anti-DNase I Polyclonal Antibody

😃Good
Catalog No. TMAB-00559
Alias DRNI, DNL1, deoxyribonuclease I

Anti-DNase I Polyclonal Antibody is a Rabbit antibody targeting DNase I. Anti-DNase I Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

Anti-DNase I Polyclonal Antibody

Anti-DNase I Polyclonal Antibody

😃Good
Catalog No. TMAB-00559Alias DRNI, DNL1, deoxyribonuclease I
Anti-DNase I Polyclonal Antibody is a Rabbit antibody targeting DNase I. Anti-DNase I Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Pack SizePriceAvailabilityQuantity
50 μL$2207-10 days
100 μL$3747-10 days
200 μL$5277-10 days
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Product Introduction

Bioactivity
Description
Anti-DNase I Polyclonal Antibody is a Rabbit antibody targeting DNase I. Anti-DNase I Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
AliasDRNI, DNL1, deoxyribonuclease I
Ig Type
IgG
Reactivity
Human,Rat (predicted:Mouse,Chicken,Pig,Horse,Rabbit)
Verified Activity
1. Paraformaldehyde-fixed, paraffin embedded (Rat spinal cord); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (DNase I) Polyclonal Antibody, Unconjugated (TMAB-00559) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
2. Sample:
MKN45 (Human) Cell Lysate at 30 μg
Primary: Anti-DNase I (TMAB-00559) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 29 kDa
Observed band size: 29 kDa
3. Sample:
MOLT-4 (Human) Cell Lysate at 30 μg
Primary: Anti-DNase I (TMAB-00559) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 29 kDa
Observed band size: 29 kDa
4. Paraformaldehyde-fixed, paraffin embedded (rat intestine); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (DNase I) Polyclonal Antibody, Unconjugated (TMAB-00559) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Application
WB,IHC-P,IHC-Fr,IF
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationSecreted. Nucleus envelope.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundDeoxyribonuclease I gene is approximately 3.2 kb long with 9 exons separated by 8 introns. In the form of a bovine pancreatic enzyme preparation, it occupies an important place in the history of protein chemistry and enzymology: it was the first enzyme to be recognized as specific for DNA; it was the first DNase to be crystallized; and it was the first DNase for which a specific protein inhibitor was characterized. DNase I is a Ca2+ and Mg2+ dependant endonuclease. DNase I is synthesized in the pancreas and stored in zymogen granules. It has been used to reduce the viscosity of cystic fibrosis sputum. A DNase I-like enzyme appears to catalyze the degradation of chromatin to oligo- and mononucleosomes during apoptosis. A recent study has demonstrated an endonuclease with activity and antigenicity indistinguishable from DNase I in thymocytes, cells susceptible to apoptosis. DNase I is an endonuclease that hydrolyzes double-stranded or single stranded DNA preferentially at sites adjacent to pyrimidine nucleotides. The product of hydrolysis is a complex mixture of 5'-phosphate mononucleotides and oligonucleotides. In the presence of Mg ion, DNase I attacks each strand of DNA independently and the cleavage sites are random.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human DNase I
Antigen Species
Human
Gene ID
Uniprot ID
Biology Area
Metabolism,DNAses,DNAses,Nuclear,Apoptosis
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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