Anti-DNA Polymerase epsilon p59 Polyclonal Antibody is a Rabbit antibody targeting DNA Polymerase epsilon p59. Anti-DNA Polymerase epsilon p59 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.

IgG

Human,Mouse (predicted:Rat,Cow,Horse,Sheep)

1. Tissue/cell: human laryngo carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-POLE2 Polyclonal Antibody, Unconjugated (TMAB-05217) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
2. Tissue/cell: Mouse embryos tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-DNA Polymerase epsilon p59 Polyclonal Antibody, Unconjugated (TMAB-05217) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining

IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human DNA Polymerase epsilon p59

Human

POLE2

DNA polymerase epsilon subunit 2

Participates in DNA repair and in chromosomal DNA replication.