Anti-DNA Ligase IV Antibody (5M82) is a Rabbit antibody targeting DNA Ligase IV. Anti-DNA Ligase IV Antibody (5M82) can be used in IF, IHC-Fr, IHC-P, WB.

IgG

5M82

Human

1. Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with DNA Ligase IV Monoclonal Antibody, Unconjugated (TMAB-05212) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded Human Lymph; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with DNA Ligase IV Monoclonal Antibody, Unconjugated (TMAB-05212) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with DNA Ligase IV Monoclonal Antibody, Unconjugated (TMAB-05212) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
4. 25 ug total protein per lane of various lysates (see on figure) probed with DNA Ligase IV monoclonal antibody, unconjugated (TMAB-05212) at 1: 1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.

IF=1:50-200; IHC-Fr=1:50-200; IHC-P=1:50-200; WB=1:500-2000

Monoclonal

Recombinant Protein: human DNA Ligase IV

Human

LIG4

DNA ligase 4

Efficiently joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The LIG4-XRCC4 complex is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of LIG4. Binding of the LIG4-XRCC4 complex to DNA ends is dependent on the assembly of the DNA-dependent protein kinase complex DNA-PK to these DNA ends.