Anti-Dlx5 Antibody (8Z604) is a Rabbit antibody targeting Dlx5. Anti-Dlx5 Antibody (8Z604) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF,IP.

IgG

8Z604

Human,Mouse,Rat

1. Western blot analysis of (1) HeLa cell lysate; (2) RAW264.7 cell lysate. Using Dlx5 (TMAB-05198) monoclonal antibody at 1: 1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
2. 4% Paraformaldehyde-fixed U-87 MG (H) cell; Triton X-100 at r. T. for 20 min; Antibody incubation with (Dlx5) monoclonal Antibody, unconjugated (TMAB-05198) 1: 100, 90 min at 37°C; followed by BF488 conjugated Goat Anti-Rabbit IgG antibody (green, BF488) at 37°C for 90 min, DAPI (blue) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.

WB: 1:1000-2000; IHC-P: 1:100-200; IHC-Fr: 1:100-200; ICC/IF: 1:50-200; IF: 1:50-200; IP: 1:20-50

Monoclonal

A synthesized peptide: human DLX5

Human

DLX5

Homeobox protein DLX-5

Transcriptional factor involved in bone development. Acts as an immediate early BMP-responsive transcriptional activator essential for osteoblast differentiation. Stimulates ALPL promoter activity in a RUNX2-independent manner during osteoblast differentiation. Stimulates SP7 promoter activity during osteoblast differentiation. Promotes cell proliferation by up-regulating MYC promoter activity. Involved as a positive regulator of both chondrogenesis and chondrocyte hypertrophy in the endochondral skeleton. Binds to the homeodomain-response element of the ALPL and SP7 promoter. Binds to the MYC promoter. Requires the 5'-TAATTA-3' consensus sequence for DNA-binding.