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Anti-DLST Polyclonal Antibody

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Catalog No. TMAB-00555
Alias DLTS, dihydrolipoamide S-succinyltransferase (E2 component of 2-oxo-glutarate complex)

Anti-DLST Polyclonal Antibody is a Rabbit antibody targeting DLST. Anti-DLST Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,ELISA.

Anti-DLST Polyclonal Antibody

Anti-DLST Polyclonal Antibody

😃Good
Catalog No. TMAB-00555Alias DLTS, dihydrolipoamide S-succinyltransferase (E2 component of 2-oxo-glutarate complex)
Anti-DLST Polyclonal Antibody is a Rabbit antibody targeting DLST. Anti-DLST Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,ELISA.
Pack SizePriceAvailabilityQuantity
50 μL$2227-10 days
100 μL$3737-10 days
200 μL$5277-10 days
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Product Introduction

Bioactivity
Description
Anti-DLST Polyclonal Antibody is a Rabbit antibody targeting DLST. Anti-DLST Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,ELISA.
AliasDLTS, dihydrolipoamide S-succinyltransferase (E2 component of 2-oxo-glutarate complex)
Ig Type
IgG
Reactivity
Human,Mouse,Rat (predicted:Dog,Pig,Cow,Horse,Rabbit,Sheep)
Verified Activity
1. Paraformaldehyde-fixed, paraffin embedded (mouse heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (DLST) Polyclonal Antibody, Unconjugated (TMAB-00555) at 1:100 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded (rat heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (DLST) Polyclonal Antibody, Unconjugated (TMAB-00555) at 1:100 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded (human heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (DLST) Polyclonal Antibody, Unconjugated (TMAB-00555) at 1:100 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded (rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (DLST) Polyclonal Antibody, Unconjugated (TMAB-00555) at 1:100 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded (human liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (DLST) Polyclonal Antibody, Unconjugated (TMAB-00555) at 1:100 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
6. Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (DLST) Polyclonal Antibody, Unconjugated (TMAB-00555) at 1:100 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
7. Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (DLST) Polyclonal Antibody, Unconjugated (TMAB-00555) at 1:100 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
8. Paraformaldehyde-fixed, paraffin embedded (human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (DLST) Polyclonal Antibody, Unconjugated (TMAB-00555) at 1:100 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
9. Paraformaldehyde-fixed, paraffin embedded (mouse kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (DLST) Polyclonal Antibody, Unconjugated (TMAB-00555) at 1:100 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
10. Paraformaldehyde-fixed, paraffin embedded (human testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (DLST) Polyclonal Antibody, Unconjugated (TMAB-00555) at 1:100 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
11. Paraformaldehyde-fixed, paraffin embedded (human skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (DLST) Polyclonal Antibody, Unconjugated (TMAB-00555) at 1:100 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
12. Paraformaldehyde-fixed, paraffin embedded (mouse skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (DLST) Polyclonal Antibody, Unconjugated (TMAB-00555) at 1:100 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
13. Sample:
Lane 1: Mouse Liver tissue lysates
Lane 2: Mouse Muscle tissue lysates
Lane 3: Mouse Testis tissue lysates
Lane 4: Rat Heart tissue lysates
Lane 5: Rat Muscle tissue lysates
Primary: Anti-DLST (TMAB-00555) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 41 kDa
Observed band size: 46 kDa
Application
WB,IHC-P,IHC-Fr,IF,ELISA
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; ELISA: 1:5000-10000
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationMitochondrion.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundThe 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO2. The complex contains multiple copies of three enzymatic components: 2-oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2) and lipoamide dehydrogenase (E3). DLST (dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex, mitochondrial), also known as DLTS or 2-oxoglutarate dehydrogenase complex component E2, is a 453 amino acid protein belonging to the 2-oxoacid dehydrogenase family. DLST covalently binds one lipoyl cofactor and participates in L-lysine degradation via the saccharopine pathway. Localized to the mitochondrion, DLST forms a 24-polypeptide structural core with octahedral symmetry. The gene encoding DLST maps to human chromosome 14q24.3 and mouse chromosome 12 D2.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human DLST
Antigen Species
Human
Gene ID
Uniprot ID
Biology Area
Hydrolysis,Amino acid metabolism,Energy Metabolism,Mitochondrial markers,Cancer,Amino Acids,Energy Metabolism,Mitochondrial
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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