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Anti-DLK1 Polyclonal Antibody
😃Good
Catalog No. TMAB-00553
Alias ZOG, secredeltin, Pref1, pG2, FA1, DLK-1, DLK1, DLK
Anti-DLK1 Polyclonal Antibody is a Rabbit antibody targeting DLK1. Anti-DLK1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-DLK1 Polyclonal Antibody
Anti-DLK1 Polyclonal Antibody
😃Good
Catalog No. TMAB-00553Alias ZOG, secredeltin, Pref1, pG2, FA1, DLK-1, DLK1, DLK
Anti-DLK1 Polyclonal Antibody is a Rabbit antibody targeting DLK1. Anti-DLK1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $221 | 7-10 days | |
| 100 μL | $373 | 7-10 days | |
| 200 μL | $528 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-DLK1 Polyclonal Antibody is a Rabbit antibody targeting DLK1. Anti-DLK1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Alias | ZOG, secredeltin, Pref1, pG2, FA1, DLK-1, DLK1, DLK |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat (predicted:Rabbit,Sheep) |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (DLK1) Polyclonal Antibody, Unconjugated (TMAB-00553) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 2. Sample: Lane 1: Mouse Cerebrum tissue lysates Lane 2: Rat Cerebrum tissue lysates Primary: Anti-DLK1 (TMAB-00553) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 42 kDa Observed band size: 45 kDa |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Membrane; Single-pass type I membrane protein. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | This gene encodes a transmembrane protein containing six epidermal growth factor repeats. The protein is involved in the differentiation of several cell types, including adipocytes; it is also thought to be a tumor suppressor. It is one of several imprinted genes located in a region of on chr 14q32. Certain mutations in this imprinted region can cause phenotypes similar to maternal and paternal uniparental disomy of chromosome 14 (UPD14). This gene is expressed from the paternal allele. A polymorphism within this gene has been associated with child and adolescent obesity. The mode of inheritance for this polymorphism is polar overdominance; this non-Mendelian inheritance pattern was first described in sheep with the callipyge phenotype, which is characterized by muscle hypertrophy and decreased fat mass. [provided by RefSeq, Mar 2010]. |
| Immunogen | KLH conjugated synthetic peptide: sheep DLK1 |
| Antigen Species | Sheep |
| Biology Area | Hypothalamic pituitary adrenal axis,Neurogenesis,Calcium Binding Proteins,Surface Molecules |
| Molecular Weight | Theoretical: 42 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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