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Anti-DLK1 Polyclonal Antibody

😃Good
Catalog No. TMAB-00553
Alias ZOG, secredeltin, Pref1, pG2, FA1, DLK-1, DLK1, DLK

Anti-DLK1 Polyclonal Antibody is a Rabbit antibody targeting DLK1. Anti-DLK1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

Anti-DLK1 Polyclonal Antibody

Anti-DLK1 Polyclonal Antibody

😃Good
Catalog No. TMAB-00553Alias ZOG, secredeltin, Pref1, pG2, FA1, DLK-1, DLK1, DLK
Anti-DLK1 Polyclonal Antibody is a Rabbit antibody targeting DLK1. Anti-DLK1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Pack SizePriceAvailabilityQuantity
50 μL$2217-10 days
100 μL$3737-10 days
200 μL$5287-10 days
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Product Introduction

Bioactivity
Description
Anti-DLK1 Polyclonal Antibody is a Rabbit antibody targeting DLK1. Anti-DLK1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
AliasZOG, secredeltin, Pref1, pG2, FA1, DLK-1, DLK1, DLK
Ig Type
IgG
Reactivity
Human,Mouse,Rat (predicted:Rabbit,Sheep)
Verified Activity
1. Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (DLK1) Polyclonal Antibody, Unconjugated (TMAB-00553) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
2. Sample:
Lane 1: Mouse Cerebrum tissue lysates
Lane 2: Rat Cerebrum tissue lysates
Primary: Anti-DLK1 (TMAB-00553) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 42 kDa
Observed band size: 45 kDa
Application
WB,IHC-P,IHC-Fr,IF
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationMembrane; Single-pass type I membrane protein.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundThis gene encodes a transmembrane protein containing six epidermal growth factor repeats. The protein is involved in the differentiation of several cell types, including adipocytes; it is also thought to be a tumor suppressor. It is one of several imprinted genes located in a region of on chr 14q32. Certain mutations in this imprinted region can cause phenotypes similar to maternal and paternal uniparental disomy of chromosome 14 (UPD14). This gene is expressed from the paternal allele. A polymorphism within this gene has been associated with child and adolescent obesity. The mode of inheritance for this polymorphism is polar overdominance; this non-Mendelian inheritance pattern was first described in sheep with the callipyge phenotype, which is characterized by muscle hypertrophy and decreased fat mass. [provided by RefSeq, Mar 2010].
Antigen Details
Immunogen
KLH conjugated synthetic peptide: sheep DLK1
Antigen Species
Sheep
Biology Area
Hypothalamic pituitary adrenal axis,Neurogenesis,Calcium Binding Proteins,Surface Molecules
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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