Anti-DIS3L2 Polyclonal Antibody is a Rabbit antibody targeting DIS3L2. Anti-DIS3L2 Polyclonal Antibody can be used in FCM.

IgG

Mouse (predicted:Human,Rat,Dog,Pig,Cow,Sheep)

Blank control: Mouse kidney.
Primary Antibody (green line): Rabbit Anti-DIS3L2 antibody (TMAB-05168)
Dilution: 2 μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-AF488
Dilution: 1 μg /test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

FCM: 2μg/Test

Polyclonal

KLH conjugated synthetic peptide: human DIS3L2

Human

DIS3L2

DIS3-like exonuclease 2

Ribonuclease that plays a critical role in RNA metabolism. It is essential for correct mitosis, and negatively regulates cell proliferation.