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Anti-DHX36 Polyclonal Antibody
Catalog No. TMAB-05135 Copy Product Info
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Anti-DHX36 Polyclonal Antibody is a Rabbit antibody targeting DHX36. Anti-DHX36 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-DHX36 Polyclonal Antibody
Copy Product Info🥰Excellent
Catalog No. TMAB-05135
Anti-DHX36 Polyclonal Antibody is a Rabbit antibody targeting DHX36. Anti-DHX36 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-DHX36 Polyclonal Antibody
| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 50 μL | $223 | 7-10 days | 7-10 days | |
| 100 μL | $371 | 7-10 days | 7-10 days | |
| 200 μL | $528 | 7-10 days | 7-10 days |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Antigen Details
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| Description | Anti-DHX36 Polyclonal Antibody is a Rabbit antibody targeting DHX36. Anti-DHX36 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| Reactivity | Mouse,Rat (predicted:Human,Cow,Horse,Rabbit,Sheep) |
| Verified Activity | 1. Sample: Cerebrum (Mouse) Lysate at 40 μg Primary: Anti-DHX36 (TMAB-05135) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 115 kD Observed band size: 115 kD 2. Sample: Testis (Mouse) Lysate at 40 μg Primary: Anti-DHX36 (TMAB-05135) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 115 kD Observed band size: 115 kD 3. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (DHX36) Polyclonal Antibody, Unconjugated (TMAB-05135) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (DHX36) Polyclonal Antibody, Unconjugated (TMAB-05135) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining. 5. Paraformaldehyde-fixed, paraffin embedded (Rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (DHX36) Polyclonal Antibody, Unconjugated (TMAB-05135) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Nucleus. Cytoplasm. Isoform 1 preferentially localized to the nucleus and isoform 2 localized to the cytoplasm. However, partitioning of cellular localization between the nucleus and cytoplasm is not exclusive, as isoform 1 was also detected in the cytoplasm. Both isoforms were excluded from nucleoli. |
| Tissue Specificity | Highly expressed in testis. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | This gene is a member of the DEAH-box family of RNA-dependent NTPases which are named after the conserved amino acid sequence Asp-Glu-Ala-His in motif II. The protein encoded by this gene has been shown to enhance the deadenylation and decay of mRNAs with 3'-UTR AU-rich elements (ARE-mRNA). The protein has also been shown to resolve into single strands the highly stable tetramolecular DNA configuration (G4) that can form spontaneously in guanine-rich regions of DNA. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008] |
| Immunogen | KLH conjugated synthetic peptide: human DHX36 |
| Antigen Species | Human |
| Gene Name | DHX36 |
| Gene ID | |
| Protein Name | ATP-dependent DNA/RNA helicase DHX36 |
| Uniprot ID | |
| Function | Plays a role in degradation and deadenylation of mRNAs containing in their 3'-UTR the consensus ARE sequence element. May function in sex development and spermatogenesis. |
| Molecular Weight | Theoretical: 115 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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