Anti-DHRS2 Polyclonal Antibody is a Rabbit antibody targeting DHRS2. Anti-DHRS2 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Human,Mouse,Rat

1. Tissue/cell: rat kidney tissue; 4% Paraformaldehyde-fixed and paraffin-Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-DHRS Polyclonal Antibody, Unconjugated (TMAB-05130) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
2. Tissue/cell: rat kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-DHRS Polyclonal Antibody, Unconjugated (TMAB-05130) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
3. Protein: liver (mouse) lysates at 30 μg;
Primary: Anti-DHRS2 (TMAB-05130) at 1:300;
Secondary: HRP conjugated Goat-Anti-Rabbit IgGat 1: 3000;
ECL excitated the fluorescence;
Predicted band size: 27 kD
Observed band size: 27/75 kD
4. Protein: lung (mouse) lysates at 40 μg;
Primary: Anti-DHRS2 (TMAB-05130) at 1:300;
Secondary: HRP conjugated Goat-Anti-Rabbit IgG at 1: 5000;
ECL excitated the fluorescence;
Predicted band size: 27 kD
Observed band size: 27 kD
5. Protein: Hela (Human) lysates at 40 μg;
Primary: rabbit Anti-DARC (TMAB-05130) at 1:300;
Secondary: HRP conjugated Goat-Anti-rabbit IgG at 1: 5000;
Predicted band size: 27 kD
Observed band size: 30 kD

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human DHRS2

Human

DHRS2

Dehydrogenase/reductase SDR family member 2, mitochondrial

Displays NADPH-dependent dicarbonyl reductase activity in vitro with 3,4-Hexanedione, 2,3-Heptanedione and 1-Phenyl-1,2-propanedione as substrates. No reductase activity is displayed in vitro with steroids, retinoids and sugars as substrates. May inhibit cell replication.