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Anti-DHFR Polyclonal Antibody
😃Good
Catalog No. TMAB-05123
Anti-DHFR Polyclonal Antibody is a Rabbit antibody targeting DHFR. Anti-DHFR Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.
Anti-DHFR Polyclonal Antibody
Anti-DHFR Polyclonal Antibody
😃Good
Catalog No. TMAB-05123
Anti-DHFR Polyclonal Antibody is a Rabbit antibody targeting DHFR. Anti-DHFR Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $220 | 7-10 days | 7-10 days | |
| 100 μL | $373 | 7-10 days | 7-10 days | |
| 200 μL | $529 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-DHFR Polyclonal Antibody is a Rabbit antibody targeting DHFR. Anti-DHFR Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| Reactivity | Human,Mouse |
| Application | |
| Recommended Dose | IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:50-200 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Isoform Alpha: Mitochondrion membrane; Single-pass membrane protein. Cytoplasm. |
| Tissue Specificity | Widely expressed in fetal and adult tissues, including throughout the fetal and adult brains and whole blood. Expression is higher in the adult brain than in the fetal brain. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | DHFR catalyzes the NADPH-dependent reduction of dihydrofolate to tetrahydrofolate, and is a crucial enzyme for the synthesis of purines, pyrimidines and some amino acids. Inhibition of the activity of this enzyme leads to arrest of DNA synthesis and cell death. Gene expression of methotrexate (MTX)-resistant variants of DHFR in normal hematopoietic cells is a potential strategy to permit administration of larger doses of MTX by alleviating drug toxicity in normal cells and tissues that are drug sensitive. |
| Immunogen | KLH conjugated synthetic peptide: human DHFR |
| Antigen Species | Human |
| Gene Name | DHFR |
| Gene ID | |
| Protein Name | Dihydrofolate reductase |
| Uniprot ID | |
| Function | Key enzyme in folate metabolism. Contributes to the de novo mitochondrial thymidylate biosynthesis pathway. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis. Binds its own mRNA and that of DHFRL1. |
| Molecular Weight | Theoretical: 21 kDa. |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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Tech Support
Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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