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Anti-DHFR Antibody (5H593)

Catalog No. TMAB-05124 Copy Product Info
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Anti-DHFR Antibody (5H593) is a Rabbit antibody targeting DHFR. Anti-DHFR Antibody (5H593) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.

Anti-DHFR Antibody (5H593)

Copy Product Info
🥰Excellent
Catalog No. TMAB-05124

Anti-DHFR Antibody (5H593) is a Rabbit antibody targeting DHFR. Anti-DHFR Antibody (5H593) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.

Anti-DHFR Antibody
(5H593)
Pack SizePriceUSA StockGlobal StockQuantity
25 μL$1487-10 days7-10 days
50 μL$2647-10 days7-10 days
100 μL$4737-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Description
Anti-DHFR Antibody (5H593) is a Rabbit antibody targeting DHFR. Anti-DHFR Antibody (5H593) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.
Ig Type
IgG
Clone
5H593
Reactivity
Human,Mouse,Rat
Verified Activity
25 μg total protein per lane of various lysates (see on figure) probed with DHFR monoclonal antibody, unconjugated (TMAB-05124) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
Application
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-200; IHC-Fr: 1:100-200; ICC/IF: 1:50-200; IF: 1:50-200
Antibody Type
Monoclonal
Host SpeciesRabbit
Subcellular LocalizationIsoform Alpha: Mitochondrion membrane; Single-pass membrane protein. Cytoplasm.
Tissue SpecificityWidely expressed in fetal and adult tissues, including throughout the fetal and adult brains and whole blood. Expression is higher in the adult brain than in the fetal brain.
ConstructionRecombinant Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation10mM phosphate buffered saline (pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.
Research BackgroundKey enzyme in folate metabolism. Contributes to the de novo mitochondrial thymidylate biosynthesis pathway. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis. Binds its own mRNA and that of DHFR2.
Antigen Details
Immunogen
A synthesized peptide: human Dihydrofolate reductase
Antigen Species
Human
Gene Name
DHFR
Gene ID
Protein Name
Dihydrofolate reductase
Uniprot ID
Function
Key enzyme in folate metabolism. Contributes to the de novo mitochondrial thymidylate biosynthesis pathway. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis. Binds its own mRNA and that of DHFRL1.
Chemical Properties
Molecular WeightTheoretical: 21 kDa. Actual: 21 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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