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Anti-DGKA Polyclonal Antibody
Catalog No. TMAB-05114 Copy Product Info
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Anti-DGKA Polyclonal Antibody is a Rabbit antibody targeting DGKA. Anti-DGKA Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-DGKA Polyclonal Antibody
Copy Product Info🥰Excellent
Catalog No. TMAB-05114
Anti-DGKA Polyclonal Antibody is a Rabbit antibody targeting DGKA. Anti-DGKA Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-DGKA Polyclonal Antibody
| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 50 μL | $220 | 7-10 days | 7-10 days | |
| 100 μL | $372 | 7-10 days | 7-10 days | |
| 200 μL | $527 | 7-10 days | 7-10 days |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
Stability & Storage
| Description | Anti-DGKA Polyclonal Antibody is a Rabbit antibody targeting DGKA. Anti-DGKA Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| Reactivity | Human,Rat (predicted:Mouse,Dog,Pig,Cow,Horse,Rabbit,Sheep) |
| Verified Activity | 1. Sample: Hela Cell (Human) Lysate at 40 μg Primary: Anti-DGKA (TMAB-05114) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 83 kD Observed band size: 83 kD 2. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (DGKA) Polyclonal Antibody, Unconjugated (TMAB-05114) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Tissue Specificity | Lymphocytes and oligodendroglial cells. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | The protein encoded by this gene belongs to the eukaryotic diacylglycerol kinase family. It acts as a modulator that competes with protein kinase C for the second messenger diacylglycerol in intracellular signaling pathways. It also plays an important role in the resynthesis of phosphatidylinositols and phosphorylating diacylglycerol to phosphatidic acid. Alternative splicing occurs at this locus and four transcript variants encoding the same protein have been identified. [provided by RefSeq, Jul 2008] |
| Immunogen | KLH conjugated synthetic peptide: human DGKA |
| Antigen Species | Human |
| Gene Name | DGKA |
| Gene ID | |
| Protein Name | Diacylglycerol kinase alpha |
| Uniprot ID | |
| Function | Upon cell stimulation converts the second messenger diacylglycerol into phosphatidate, initiating the resynthesis of phosphatidylinositols and attenuating protein kinase C activity. |
| Molecular Weight | Theoretical: 83 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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