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Anti-DFNA5 Polyclonal Antibody
😃Good
Catalog No. TMAB-00541
Alias Non-syndromic hearing impairment protein 5, Nonsyndromic hearing impairment protein, Inversely correlated with estrogen receptor expression 1, ICERE-1, GSDME, Fin15, EG14210, Dfna5h, DFNA5 gene, DFNA5, Deafness, autosomal dominant 5 protein, Deafness, autosomal dominant 5, 4932441K13Rik, 2310037D07Rik
Anti-DFNA5 Polyclonal Antibody is a Rabbit antibody targeting DFNA5. Anti-DFNA5 Polyclonal Antibody can be used in IF,IHC-Fr,IHC-P,WB.
Anti-DFNA5 Polyclonal Antibody
Anti-DFNA5 Polyclonal Antibody
😃Good
Catalog No. TMAB-00541Alias Non-syndromic hearing impairment protein 5, Nonsyndromic hearing impairment protein, Inversely correlated with estrogen receptor expression 1, ICERE-1, GSDME, Fin15, EG14210, Dfna5h, DFNA5 gene, DFNA5, Deafness, autosomal dominant 5 protein, Deafness, autosomal dominant 5, 4932441K13Rik, 2310037D07Rik
Anti-DFNA5 Polyclonal Antibody is a Rabbit antibody targeting DFNA5. Anti-DFNA5 Polyclonal Antibody can be used in IF,IHC-Fr,IHC-P,WB.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $220 | 7-10 days | 7-10 days | |
| 100 μL | $372 | 7-10 days | 7-10 days | |
| 200 μL | $527 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-DFNA5 Polyclonal Antibody is a Rabbit antibody targeting DFNA5. Anti-DFNA5 Polyclonal Antibody can be used in IF,IHC-Fr,IHC-P,WB. |
| Synonyms | Non-syndromic hearing impairment protein 5, Nonsyndromic hearing impairment protein, Inversely correlated with estrogen receptor expression 1, ICERE-1, GSDME, Fin15, EG14210, Dfna5h, DFNA5 gene, DFNA5, Deafness, autosomal dominant 5 protein, Deafness, autosomal dominant 5, 4932441K13Rik, 2310037D07Rik |
| Ig Type | IgG |
| Reactivity | Mouse,Rat (predicted:Human,Dog,Cow,Sheep) |
| Verified Activity | 1. Sample: Small intestine (Mouse) Lysate at 40 μg Primary: Anti-DFNA5 (TMAB-00541) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 54 kDa Observed band size: 54 kDa 2. Protein: intestinal (mouse) lysate at 40 μg; Primary: rabbit Anti-DFNA5 (TMAB-00541) at 1:300; Secondary: HRP conjugated Goat-Anti-rabbit Igg (secondary antibody) at 1: 5000; Predicted band size: 54 kDa Observed band size: 54 kDa 3. Paraformaldehyde-fixed, paraffin embedded (rat spleen tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (DFNA5) Polyclonal Antibody, Unconjugated (TMAB-00541) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining.    |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Tissue Specificity | Expressed in cochlea. Low level of expression in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas, with highest expression in placenta. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1 mg/mL |
| Research Background | Hearing impairment is a heterogeneous condition with over 40 loci described. The protein encoded by this gene is expressed in fetal cochlea, however, its function is not known. Nonsyndromic hearing impairment is associated with a mutation in this gene. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| Immunogen | KLH conjugated synthetic peptide: human DFNA5 |
| Antigen Species | Human |
| Gene Name | GSDME |
| Gene ID | |
| Protein Name | Gasdermin-E |
| Uniprot ID | |
| Biology Area | Auditory system |
| Molecular Weight | Theoretical: 54 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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