Anti-DFFA Polyclonal Antibody is a Rabbit antibody targeting DFFA. Anti-DFFA Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Human,Mouse,Rat

1. Sample:
liver (mouse) Lysate at 40 μg
lung (mouse) Lysate at 40 μg
BRL-3A (rat) Cell Lysate at 40 μg
Primary: Anti-ICAD (TMAB-05108) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 36 kD
Observed band size: 36 kD
2. Tissue/cell: human cervical carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-ICAD/DFF-45 Beta Polyclonal Antibody, Unconjugated (TMAB-05108) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
3. Tissue/cell: Human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-ICAD Polyclonal Antibody, Unconjugated (TMAB-05108) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
4. Sample: A431 Cell (Human) Lysate at 40 μg
Primary: Anti-ICAD (TMAB-05108) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 36 kD
Observed band size: 36 kD

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human ICAD

Human

DFFA

DNA fragmentation factor subunit alpha

Inhibitor of the caspase-activated DNase (DFF40).