Anti-Desmoglein 2 Antibody (4J535) is a Rabbit antibody targeting Desmoglein 2. Anti-Desmoglein 2 Antibody (4J535) can be used in FCM, ICC/IF, WB.

IgG

4J535

Human

1. 4% Paraformaldehyde-fixed HepG2 (H) cell; Triton X-100 at r. T. for 20 min; Antibody incubation with (Desmoglein 2) monoclonal Antibody, unconjugated (TMAB-05101) 1: 100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, BF488) at 37°C for 90 min, DAPI (blue) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
2. The HepG2 (H) cells were fixed with 4% PFA (10 min at r. T.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.). Primary Antibody (green): Rabbit Anti-Desmoglein 2 antibody (TMAB-05101, 1: 100); Secondary Antibody (white blue): Goat anti-Rabbit IgG-BF488 (BF488): 1 μg/test. Blank control (black): PBS. Acquisition of 20,000 events was performed.
3. 25 ug total protein per lane of various lysates (see on figure) probed with Desmoglein 2 monoclonal antibody, unconjugated (TMAB-05101) at 1: 1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.

FCM=1:50-100; ICC/IF=1:50-200; WB=1:500-2000

Monoclonal

Recombinant Protein: protein within C-terminal human Desmoglein 2

Human

DSG2

Desmoglein-2

Component of intercellular desmosome junctions. Involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion.