Anti-Desmocollin 2+3 Polyclonal Antibody is a Rabbit antibody targeting Desmocollin 2+3. Anti-Desmocollin 2+3 Polyclonal Antibody can be used in FCM.

IgG

Human (predicted:Mouse,Rat,Horse)

Blank control: K562.
Primary Antibody (green line): Rabbit Anti-Desmocollin 2+3 antibody (TMAB-05099) Dilution: 2 μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-PE Dilution: 1 μg /test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1%PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

FCM: 2μg/Test

Polyclonal

KLH conjugated synthetic peptide: human Desmocollin 2

Human

DSC2

Desmocollin-2

Component of intercellular desmosome junctions. Involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion. May contribute to epidermal cell positioning (stratification) by mediating differential adhesiveness between cells that express different isoforms.