Anti-DENND2D Polyclonal Antibody is a Rabbit antibody targeting DENND2D. Anti-DENND2D Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.

IgG

Human (predicted:Mouse,Rat,Dog,Pig,Cow,Horse,Rabbit,Sheep)

1. Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (DENND2D) Polyclonal Antibody, Unconjugated (TMAB-05083) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
2. Tissue/cell: human colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-DENND2D Polyclonal Antibody, Unconjugated (TMAB-05083) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining

IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human DENND2D

Human

DENND2D

DENN domain-containing protein 2D

Guanine nucleotide exchange factor (GEF) which may activate RAB9A and RAB9B. Promotes the exchange of GDP to GTP, converting inactive GDP-bound Rab proteins into their active GTP-bound form.