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Anti-Defensin alpha 5 Polyclonal Antibody

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Catalog No. TMAB-05062

Anti-Defensin alpha 5 Polyclonal Antibody is a Rabbit antibody targeting Defensin alpha 5. Anti-Defensin alpha 5 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.

Anti-Defensin alpha 5 Polyclonal Antibody

Anti-Defensin alpha 5 Polyclonal Antibody

Copy Product Info
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Catalog No. TMAB-05062
Anti-Defensin alpha 5 Polyclonal Antibody is a Rabbit antibody targeting Defensin alpha 5. Anti-Defensin alpha 5 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.
Pack SizePriceUSA WarehouseGlobal WarehouseQuantity
50 μL$2227-10 days7-10 days
100 μL$3737-10 days7-10 days
200 μL$5297-10 days7-10 days
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction

Bioactivity
Description
Anti-Defensin alpha 5 Polyclonal Antibody is a Rabbit antibody targeting Defensin alpha 5. Anti-Defensin alpha 5 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.
Ig Type
IgG
Reactivity
Human
Verified Activity
1. Paraformaldehyde-fixed, paraffin embedded (Human duodenum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (Defensin alpha 5) Polyclonal Antibody, Unconjugated (TMAB-05062) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded (human endometrial); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (Defensin alpha 5) Polyclonal Antibody, Unconjugated (TMAB-05062) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
Application
Recommended Dose
IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationSecreted. Cytoplasmic vesicle > secretory vesicle. Peptides HD5(20-94), HD5(23-94) and HD5(29-94) are found within tissues, HD5(20-94) being the predominant intracellular form. Peptides HD5(56-94) and HD5(63-94) are found in the extracellular milieu, HD5(63-94) being the most abundant form.
Tissue SpecificityPaneth cells of the small intestine (at protein level).
ConstructionPolyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Research BackgroundHas antimicrobial activity against Gram-negative and Gram-positive bacteria. Defensins are thought to kill microbes by permeabilizing their plasma membrane. All DEFA5 peptides exert antimicrobial activities, but their potency is affected by peptide processing.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human Defensin alpha 5
Antigen Species
Human
Gene Name
DEFA5
Gene ID
Protein Name
Defensin alpha 5
Uniprot ID
Function
Has antimicrobial activity against Gram-negative and Gram-positive bacteria. Defensins are thought to kill microbes by permeabilizing their plasma membrane. All DEFA5 peptides exert antimicrobial activities, but their potency is affected by peptide processing.
Chemical Properties
Molecular WeightTheoretical: 7.8 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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