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Anti-DDX4 Antibody (5D840)
😃Good
Catalog No. TMAY-00095
Alias VASA
Anti-DDX4 Antibody (5D840) is a Mouse antibody targeting DDX4. Anti-DDX4 Antibody (5D840) can be used in IHC-P,ICC/IF.
Anti-DDX4 Antibody (5D840)
Anti-DDX4 Antibody (5D840)
😃Good
Catalog No. TMAY-00095Alias VASA
Anti-DDX4 Antibody (5D840) is a Mouse antibody targeting DDX4. Anti-DDX4 Antibody (5D840) can be used in IHC-P,ICC/IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 100 μL | $199 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Antibody Type: Mouse Monoclonal Application: IHC-P, ICC/IF Reactivity: Human |
| Alias | VASA |
| Ig Type | Mouse IgG1 |
| Clone | 5D840 |
| Reactivity | Human |
| Verified Activity | 1. Immunochemical staining of human DDX4 in human testis with mouse monoclonal antibody (1:60, formalin-fixed paraffin embedded sections). 2. Immunofluorescence staining of DDX4 in hESC-H9 cells. Cells were fixed with 4% PFA, permeabilzed with 0.5% Triton X-100 in PBS,blocked with 10% serum, and incubated with mouse anti-DDX4 monoclonal antibody (dilution ratio 1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-mouse IgG secondary antibody (green). Positive staining was localized to Cytoplasm. |
| Application | IHC-P,ICC/IF |
| Recommended Dose | IHC-P: 1:50-1:200; ICC-IF: 1:20-1:100 |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Construction | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with a synthetic peptide corresponding to the C-terminus of the human DDX4. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | DDX4 (DEAD box polypeptide 4), characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), is an RNA helicase which is implicated in various cellular processes involving the alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. DDX4 is known to be a germ cell-specific protein and is used as a sorting marker of germline stem cells for the production of oocytes. The active vitamin D treatment prohibited the proliferation and invasion of ovarian cancer cells, and the expression level of a germ cell specific marker DEAD (Asp-Glu-Ala-Asp)-box helicase 4 (DDX4), which is overexpressed in ovarian cancer, was downregulated by active vitamin D treatment. Knockdown of DDX4 by siRNA could also suppress the invasive ability of ovarian cancer cells. Therefore, DDX4 may be considered as a diagnostic marker of ovarian cancer, and vitamin D may be a candidate drug for ovarian cancer therapy. That DDX4 is essential for the regulation of germ cell proliferation and differentiation across all three extant mammalian groups-eutherians, marsupials, and monotremes. |
| Conjucates | Unconjugated |
| Immunogen | A synthetic peptide: C-terminus of the human DDX4. |
| Antigen Species | Human |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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